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Complementation among cytoplasmic mutants of Neurospora crassa

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Summary

Heteroplasmons with normal growth rates are formed when the slow-growing, female fertile, group I or II extranuclear mutants of Neurospora crassa are combined by forced heterokaryosis with the female sterile, stopper mutants of group III. Different mutants from the same growth and fertility group do not complement each other, and the poky-like strains of group I do not interact synergistically with [mi-3], the only known group II mutant. The mitochondrial cytochrome system of the complementing heteroplasmons are as abnormal as the cytochrome complements of the component extranuclear mutants, indicating that defects in the electron transport system represented by those mutants are related inconsequentially to growth. The observed functional complementation indicates the expression of the mitochondrial genome is not restricted to the specific organelle of which it is a part.

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References

  • Beadle, G.W., Coonradt, V.L.: Heterocaryosis in Neurospora crassa. Genetics 29, 291 (1944).

    Google Scholar 

  • Bertrand, H., McDougall, K.J., Pittenger, T.H.: Somatic cell variation during uninterrupted growth of Neurospora in continuous growth tubes. J. gen. Microbiol. 50, 337 (1968).

    Google Scholar 

  • Bertrand, H., Pittenger, T.H.: Cytoplasmic mutants selected from continuously growing cultures of Neurospora crassa. Genetics 61, 643 (1969a).

    Google Scholar 

  • Bertrand, H., Pittenger, T.H.: Cytochrome spectra of three cytoplasmic mutants. Neurospora Newsl. 14, 6 (1969b).

    Google Scholar 

  • Bertrand, H., Pittenger, T.H.: Isolation and classification of extranuclear mutants of Neurospora crassa. Genetics, in press (1972).

  • Diacumakos, E.G., Garnjobst, L., Tatum, E.L.: A cytoplasmic character in Neurospora crassa. The role of nuclei and mitochondria. J. Cell Biol. 26, 427 (1965).

    Google Scholar 

  • Dodge, B.O.: Heterocaryotic vigor in Neurospora. Bull. Torrey bot. Club 69, 75 (1942).

    Google Scholar 

  • Eakin, R.T., Mitchell, H.K.: Alterations of the respiratory system of Neurospora crassa by the mi-1 mutation. J. Bact. 104, 74 (1970).

    Google Scholar 

  • Gowdridge, B.M.: Heterocaryons between strains of Neurospora crassa with different cytoplasms. Genetics 41, 780 (1956).

    Google Scholar 

  • Griffiths, A.J.F., Bertrand, H., Pittenger, T.H.: Cytochrome spectra of cytoplasmic mutants of Neurospora. Neurospora Newsl. 13, 16 (1968).

    Google Scholar 

  • Lambowitz, A.M., Slayman, C.W.: Cyanide-resistant respiration in Neurospora crassa. J. Bact. 108, 1087 (1971).

    Google Scholar 

  • McDougall, K.J., Pittenger, T.H.: A cytoplasmic variant of Neurospora crassa. Genetics 54, 551 (1966).

    Google Scholar 

  • Mitchell, M.B., Mitchell, H.K.: A nuclear gene suppressor of a cytoplasmically inherited character in Neurospora crassa. J. gen. Microbiol. 14, 84 (1956).

    Google Scholar 

  • Mitchell, M.B., Mitchell, H.K., Tissieres, A.: Mendelian and non-Mendelian factors affecting the cytochrome system in Neurospora crassa. Proc. nat. Acad. Sci. (Wash.) 39, 606 (1953).

    Google Scholar 

  • Pittenger, T.H.: Synergism of two cytoplasmically inherited mutants of Neurospora crassa. Proc. nat. Acad. Sci. (Wash.) 42, 747 (1956).

    Google Scholar 

  • Rifkin, M.R., Luck D.J.L.: Defective production of mitochondrial ribosomes in the poky mutant of Neurospora crassa. Proc. nat. Acad. Sci. (Wash.) 68, 287 (1971).

    Google Scholar 

  • Schonbaum, G.R., Bonner, W.D., Storey, B.T., Bahr, J.T.: Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiol. 47, 124 (1971).

    Google Scholar 

  • Srb, A.M.: Some consequences of nucleo-cytoplasmic recombinations among various Neurosporas. Cold Spr. Harb. Symp. quant. Biol. 23, 269 (1958).

    Google Scholar 

  • Wilson, J.F.: Mitochondrial transplantation studies in Neurospora crassa. XI Int. Bot. Congr. (Abstracts) p. 240 (1969).

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Authors and Affiliations

  1. Division of Biology, Kansas State University Manhattan, Kansas

    H. Bertrand & T. H. Pittenger

  2. Department of Agronomy, Kansas State University Manhattan, Kansas

    H. Bertrand & T. H. Pittenger

  3. Department of Biology, University of Saskatchewan, Regina Campus, Regina, Saskatchewan, Canada

    H. Bertrand & T. H. Pittenger

Authors
  1. H. Bertrand
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  2. T. H. Pittenger
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Communicated by F. Stahl

Contribution No. 1255 Department of Agronomy; Contribution No. 1148, Division of Biology, Kansas Agriculture Experiment Station, Manhattan, Kansas.

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Bertrand, H., Pittenger, T.H. Complementation among cytoplasmic mutants of Neurospora crassa . Molec. Gen. Genet. 117, 82–90 (1972). https://doi.org/10.1007/BF00268840

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  • DOI: https://doi.org/10.1007/BF00268840

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