Summary
The variation in sensitivity of the mitochondrial genome of Saccharomyces cerevisiae to ethidium bromide-induced petite mutation in response to changes in glucose concentration has been studied. Growth in high glucose considerably depressed the mutation rate, whilst small variations are observed in response to step-up or step-down in glucose concentration. Variations in mitochondrial DNA and respiratory activity during the mutagenic process are described. Effects of non-metabolizable sugars which repress mitochondrial biogenesis and a number of antimitochondrial drugs have been investigated. The results are discussed in terms of possible mechanisms of modulation of the mutation rates.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Azzi, A., Santato, M.: Interaction of ethidium bromide with the mitochondrial membrane: Cooperative binding and energy-linked changes. Biochem. biophys. Res. Commun 44, 211–217 (1971)
Bech-Hansen, N.T., Rank, G.H.: Cytoplasmically inherited ethidium bromide resistance in suppressive petities of Saccharomyces cerevisiae. Canad. J. Genet. Cytol. 15, 381–387 (1973)
Clark-Walker, G.D., Linnane, A.W.: In vivo differentiation of yeast cytoplasmic and mitochondrial protein synthesis with antibiotics. Biochem. biophys. Res. Commun. 25, 8–13 (1966)
De Deken, R.H.: The Crabtree effect and its relation to the petite mutation. J. gen. Microbiol. 44, 157–165 (1966)
Ephrussi, B., Hottinguer, H.: Direct demonstration of the mutagenic action of euflavine on baker's yeast. Nature (Lond.) 166, 956 (1950)
Gitler, C., Rubalcava, B., Caswell, A.: Fluorescence changes of ethidium bromide on binding to erythrocyte and mitochondrial membranes. Biochim. biophys. Acta (Amst.) 193, 479–481 (1969)
Goldring, E.S., Grossman, L.I., Krupnick, D., Cryer, D.R., Marmur, J.: The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide. J. molec. Biol. 52, 323–335 (1970)
Hammond, R.C., Wright, M., Whittaker, P.A.: Growth on galactose can prevent or delay the induction of petite mutants of Saccharomyces cerevisiae NCYC 239 by ethidium bromide. Biochem. Soc. Trans. 2, 218–220 (1974)
Hollenberg, C.P., Borst, P.: Conditions that prevent rho-induction by ethidium bromide. Biochem. biophys. Res. Commun. 45, 1250–1254 (1971)
Huang, M., Biggs, D.R., Clark-Walker, D.G., Linnane, A.W.: Chloramphenicol inhibition of the formation of particulate mitochondrial enzymes of Saccharomyces cerevisiae. Biochim. biophys. Acta (Amst.) 114, 434–436 (1966)
LePecq, J.B., Paoletti, C.: A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. J. molec. Biol. 27, 87–106 (1967)
Mahler, H.R.: Structural requirements in mitochondrial mutagenesis. J. Supramol. Struct. 1, 449–460 (1973)
Mahler, H.R., Bastos, R. de N.: Coupling between mitochondrial mutation and energy transduction. Proc. nat. Acad. Sci. (Wash.) 71, 2241–2245 (1974)
Marcovich, H.: Action of acriflavine on yeasts. (VIII) Detection of the active component and study of euflavine. Ann. Inst. Pasteur 81, 452–468 (1951)
Ogur, M., St. John, R., Nagai, S.: Tetrazolium overlay technique for population studies of respiratory deficiency in yeast. Science 125, 928–929 (1957)
Perlman, P.S., Mahler, H.R.: A premutational state induced in yeast by ethidium bromide. Biochem. biophys. Res. Commun. 44, 261–267 (1971)
Slonimski, P.P., Perrodin, G., Croft, J.H.: Ethidium bromide-induced mutation of yeast mitochondria; complete transformation of cells into respiratory deficient non-chromosomal “petites”. Biochem. biophys. Res. Commun. 30, 232–239 (1968)
Stein, E., Iditoiu, C.: Studies on the Crabtree effect in yeast. Rev. roum. Biochimie 8, 336–339 (1971)
Sueoka, N.: Variation and heterogeneity of base composition of deoxyribonucleic acids; a compilation of old and new data. J. molec. Biol. 3, 31–40 (1961)
Vidová, M., Kováč, L.: Nalidixic acid prevents the induction of yeast cytoplasmic respiratory deficient mutants by intercalating drugs. FEBS Lett. 22, 347–351 (1972)
Waring, M.: Variation of the supercoils in closed circular DNA by binding of antibiotics and drugs: Evidence for molecular models involving intercalation. J. molec. Biol. 54, 247–279 (1970)
Whittaker, P.A., Meyer, J., Hammond, R.: Petite mutation in yeast. Effects of nalidixic acid and glucose repression. Abstr. Commun. 9th Meet. Fed. Europ. Biochem. Soc. p. 458 (1974)
Whittaker, P.A., Wright, M.: Prevention by cycloheximide of peptite mutation in yeast. Biochem. biophys. Res. Commun. 48, 1455–1459 (1972)
Williamson, D.H., Moustacchi, E., Fennel, D.: A procedure for rapidly extracting and estimating the nuclear and cytoplasmic DNa components of yeast cells. Biochim. biophys. Acta (Amst.) 238, 369–374 (1971)
Wintersberger, E.: Proteinsynthese in isolierten Hefe-mitochondrien. Biochem. Z. 341, 409–419 (1965)
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Meyer, J.Z., Whittaker, P.A. Respiratory repression and the stability of the mitochondrial genome. Molec. Gen. Genet. 151, 333–342 (1977). https://doi.org/10.1007/BF00268798
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00268798