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Strains of Bacillus subtilis synthesizing elevated levels of isoleucine-valine biosynthetic enzymes

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Summary

Bacillus subtilis strains synthesizing elevated levels of isoleucine-valine biosynthetic enzymes were obtained by selecting for resistance to the isoleucine analogue ketothiaisoleucine. One class of resistant strains exhibits a wild-type growth rate at 45° C, but grows slowly at 37° C. A shift from the permissive to the restrictive temperature results in derepression of pathway enzymes and decreased specific activity of isoleucyl tRNA synthetase. The mutation responsible for elevated enzyme levels in temperature sensitive strains is carried on the same molecule of transforming DNA as ilvaC and pheA and is designated KTAR.

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References

  • Barat, M., Anagnostopoulos, C., Schneider, A. M.: Linkage relationships of genes controlling isoleucine, valine and leucine biosynthesis in Bacillus subtilis. J. Bact. 90, 357–369 (1965)

    Google Scholar 

  • Chapman, L. F.: Regulation of acetohydroxyacid synthetase in Bacillus subtilis. Molec. gen. Genet. 117, 14–18 (1972)

    Google Scholar 

  • Chapman, L. F., Nester, E. W.: Common element in the repression control of enzymes of histidine and aromatic amino acid biosynthesis in Bacillus subtilis. J. Bact. 96, 1658–1663 (1968)

    Google Scholar 

  • Dubnau, P., Smith, I., Marmur, J.: Gene conservation in Bacillus species. II. The location of genes concerned with the synthesis of ribosomal components and soluble RNA. Proc. nat. Acad. Sci. (Wash.) 54, 724–730 (1965)

    Google Scholar 

  • Friedemann, T. E., Haugen, G. E.: Pyruvic acid. II. The determination of keto acids in blood and urine. J. biol. Chem. 147, 415–442 (1943)

    Google Scholar 

  • Iaccarino, M., Berg, P.: Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyltransfer ribonucleic acid synthetase. J. Bact. 105, 527–537 (1971)

    Google Scholar 

  • Juni, E.: Mechanisms of formation of acetoin by bacteria. J. biol. Chem. 195, 715–726 (1952)

    Google Scholar 

  • Juni, E., Heym, G. A.: Properties of yeast pyruvate decarboxylases and their modification by proteolytic enzymes. I. Stability of decarboxylases from wild type and mutant strains. Arch. Biochem. Biophys. 127, 79–88 (1968)

    Google Scholar 

  • Kane, J. F., Stenmark, S. L., Calhoun, D. H., Jensen, R. A.: Metabolic interlock: The role of the subordinate type of enzyme in the regulation of a complex pathway. J. biol. Chem. 246, 4308–4318 (1971)

    Google Scholar 

  • Krampitz, L. O., Everett, J.: Synthesis of α-acetolactic acid. Arch. Biochem. Biophys. 17, 81–85 (1948)

    Google Scholar 

  • Lorence, J. H., Nester, E. W.: Multiple molecular forms of chorismate mutase in Bacillus subtilis. Biochemistry 6, 1541–1546 (1967)

    Google Scholar 

  • Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265–271 (1951)

    Google Scholar 

  • Nasser, D. S., Nester, E. W.: Aromatic amino acid biosynthesis: gene—enzyme relationships in Bacillus subtilis. J. Bact. 94, 1706–1714 (1967)

    Google Scholar 

  • Sjölander, J. R., Folkers, K., Adelberg, E. A., Tatum, E. L.: α, β, Dihydroxy-isovaleric acid and α, β, Dihydroxy-β-methylvaleric acid, precursors of valine and isoleucine. J. Amer. chem. Soc. 76, 1085–1087 (1954)

    Google Scholar 

  • Spizizen, J.: Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc. nat. Acad. Sci. (Wash.) 44, 1072–1078 (1958)

    Google Scholar 

  • Szentirmai, A., Szentirmai, M., Umbarger, H. E.: Isoleucine and valine metabolism of Escherichia coli. XVI. Biochemical properties of mutants resistant to thiaisoleucine. J. Bact. 95, 1672–1677 (1968)

    Google Scholar 

  • Szentirmai, A., Umbarger, H. E.: Isoleucine and valine metabolism of Escherichia coli. XV. Effect of thiaisoleucine. J. Bact. 95, 1666–1671 (1968)

    Google Scholar 

  • Umbarger, H. E.: In: Metabolic regulation (H. J. Vogel, ed.) p. 447–462. New York-London: Academic Press 1971

    Google Scholar 

  • Umbarger, H. E.: In: Genetics of industrial microorganisms, bacteria (Z. Vanek, Z. Hostalek and J. Cudlin, eds.), p. 195–218. Prague: Academia Pbl. 1973

    Google Scholar 

  • Ward, J. B., Zahler, S. A.: Regulation of leucine biosynthesis in Bacillus subtilis. Amer. Soc. Microbiol. Abs. 1, 173 (1972)

    Google Scholar 

  • Youatt, J., Montoya, A., Nester, E. W.: Possible inhibitory effect of teichoic acid on Bacillus subtilis transfer ribonucleic acid. Biochem. J. 119, 317–320 (1970)

    Google Scholar 

  • Zubay, G.: The isolation and fractionation of soluble ribonucleic acid. J. molec. Biol. 4, 347–358 (1962)

    Google Scholar 

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  1. Microbiology Section, Division of Biological Sciences, University of Missouri, Columbia, Missouri, U.S.A.

    L. F. Chapman & C. J. Hull

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  1. L. F. Chapman
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  2. C. J. Hull
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Communicated by B. A. Bridges

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Chapman, L.F., Hull, C.J. Strains of Bacillus subtilis synthesizing elevated levels of isoleucine-valine biosynthetic enzymes. Molec. Gen. Genet. 129, 87–95 (1974). https://doi.org/10.1007/BF00268623

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  • DOI: https://doi.org/10.1007/BF00268623

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