Abstract
Plant tissue cultures from various species were stored at low temperatures or under mineral oil overlay for 4 to 6 months without subcultures. After transfer to normal culture conditions, it was checked, with 3 strains, that growth characteristics and secondary metabolite production were preserved. The storage with a mineral oil overlay (easy to run and economical method) could be a possible alternative to cryogenic or low temperature storage for a large number of strains.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- DCMU:
-
3-(3,4-dichlorophenyl)-1, 1-dimethylurea
- TLC:
-
Thin layer chromatography
- HPLC:
-
High performance liquid chromatography
- CAS:
-
Ceric ammonium sulfate
References
Bridgen HP, Staby GL (1984) in Evans D.A., Sharp W.R., Ammirato P.V., Yamada Y.: Handbook of plant cell culture, volume 1, Macmillan, New-York: 816–827.
Caplin SM (1959) Am J Botany, 46: 324–329
Chen THH, Kartha KK, Constabel F, Gusta LV (1984) Plant Physiol., 75: 720–725
Dougall KD (1980) in Staba J.: Plant tissue culture as a source of biochemicals, CRC Press, Inc Boca Raton, Florida: 115–122
Farnsworth NR, Blomster RN, Damratoskid D, Meer WA, Cammarato LV (1964) Lloydia, 27: 302–314.
Frischnecht PM, Baumann TW (1980) Planta Medica, 40 245–249
Gamborg OL, Miller RA, Ojima K (1968) Exp cell Res 50: 151–158
Hiraoka N, Kodama T (1982) in Fujiwara A.: Plant tissue culture, Proc Intl Cong Plant Tissue and cell culture, Tokyo: 359–360
Hiraoka N, Kodama T (1984) Plant cell tissue organ culture, 3: 349–357
Kuble LS (1927) The solubility of O2, CO2, N2 in mineral oil and the transfer of CO2 from oil to air. Journ Biol Chem 72: 545–548
Larkin PJ, Brettel R, Ryan S, Scowcroft W (1983) in Potrykus I, Harms CT, Hinnen A., Hutter R, King PJ, Shillito RD: Protoplasts, 6th International Protoplast Symposium, Basel: 51–56
Meins F Jr (1983) Ann Rev Plant Physiol., 34: 327–346
Nitzche W (1984) in Evans DA, Sharp WR, Ammirato PV, Yamada Y: Handbook of plant cell culture, volume 1, Macmillan, New-York: 782–805
Paris RR, Cosson L (1965) CR Acad Sc, Série D, 260 3148–3159
Rose D, Martin SM (1975) Can J Bot, 53: 315–320
Vernon LP (1960) Analytical Chemistry 32: 1144–1150
Withers LA (1978) in Thorpe TA: Frontiers of plant tissue culture. International Association for plant tissue culture, Calgary: 297–306
Withers LA (1982) in Fujiwara A: Plant tissue culture. Proc Intl Cong Plant tissue and cell culture, Tokyo: 793–794
Withers LA (1983) in Mantell SH, Smith H: Plant Biotechnology, volume 18, Cambridge University Press, Cambridge: 187–218
Yatazawa M (1982) in: Fujiwara A (Ed) Plant tissue culture, 1982, Proc Intl Cong Plant tissue and cell culture, Tokyo: 261–262.
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Communicated by P. J. King
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Augereau, J.M., Courtois, D. & Petiard, V. Long term storage of callus cultures at low temperatures or under mineral oil layer. Plant Cell Reports 5, 372–376 (1986). https://doi.org/10.1007/BF00268605
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DOI: https://doi.org/10.1007/BF00268605