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Induction and differentiation of callus from embryos of Cocos nucifera L. by IAA-conjugates

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Abstract

Calli from young embryos of Cocos nucifera L. were induced on B5 medium supplemented with IAA-conjugates (IAA-asp or IAA-ala) at a concentration of 2.0 mg/1 and callusing was increased by about 10% if both IAA-conjugates, IAA-asp and IAA-ala were added together. Differentiation of shoots and roots was achieved by transferring calli to B5 medium supplemented with either IAA-asp (2.0 mg/1)+Kn(2.0 mg/1) or NAA (2.0 mg/1). Complete plantlets were obtained on B5 medium supplemented with NAA (0.5 mg/1)+BAP (2.0 mg/1)+PVP (1.0 g/1).

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Abbreviations

IAA:

Indole-3-acetic acid

IAA-ala:

Indole acetyl-L-alanine

IAA-asp:

Indole acetyl-L-aspartic acid

Kn:

Kinetin

BAP:

N6-benzylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

NAA:

α-naphthalene acetic acid

PVP:

Polyvinylpyrolidone

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Authors and Affiliations

  1. Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India

    Neera Bhalla-Sarin, Suman Bagga, Sudhir K. Sopory & Sipra Guha-Mukherjee

Authors
  1. Neera Bhalla-Sarin
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  2. Suman Bagga
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  3. Sudhir K. Sopory
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  4. Sipra Guha-Mukherjee
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Additional information

Communicated by I. K. Vasil

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Bhalla-Sarin, N., Bagga, S., Sopory, S.K. et al. Induction and differentiation of callus from embryos of Cocos nucifera L. by IAA-conjugates. Plant Cell Reports 5, 322–324 (1986). https://doi.org/10.1007/BF00268591

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  • DOI: https://doi.org/10.1007/BF00268591

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