Summary
Cycloheximide-resistant cell lines have been recovered from tissue cultures of haploid Nicotiana tabacum at the usual mutant frequency (10-6). One such line, CR 4/6 was studied in detail. Resistance is based on the potential of the callus, to inactive cycloheximide. The transient nature of the resistance and its association with differentiated cells indicate that the new phenotype is not due to a mutation, but rather to an alteration in gene expression.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Carlson, P.S.: Methionine-sulfoximine-resistant mutants of tobacco. Science 180, 1366–1368 (1973)
Cocking, E.C., Power, J.B., Evans, P.K., Safwat, F., Frearson, E.M., Hayward, S.F., Berry, S.F., George, D.: Naturally occurring differential drug sensitivities of cultured plant protoplasts. Plant Sci. Letters 3, 341–350 (1974)
Farkas, G.L., Stahmann, M.A.: On the nature of changes in peroxidasc isoezymes in bean leaves infected by southern bean mosaic virus. Phytopathology 56, 669–677 (1966)
Hart, M.A., Tyson, H., Blomberg, R.: Measurement of activity of peroxidase isoenzymes in flax (Linum usitatissimum) Canad. J. Bot. 49, 2129–2137 (1971)
Haugli, F.B., Dove, W.F.: Genetics and biochemistry of cycloheximide resistance in Physarum polycephalum. Molec. gen. Genet. 118, 97–107 (1972)
Linsmayer, E.M., Skoog, F.: Organic growth factor requirements of tobacco tissue cultures. Physiol. Plantarum 18, 100–127 (1965)
Mäder, M., Bopp, M.: Neue Vorstellungen zum Problem der Isoperoxidasen anhand der Trennung durch Disk-Elektrophorese und isoclektrische Fokussierung. Planta (Berl.) 128, 247–253 (1976)
Maliga, P.: Isolation of mutants of cultured plant cells. In: Cell genetics of higher plants (eds. D. Dudits, G.L. Farkas, and P. Maliga) Budapest: Publishing House of the Hungarian Academy of Sciences 1976 (in press)
Maliga, P., Sz.-Breznovits, Á., Márton, L.: Strptomycin-resistant plants from callus culture of haploid tobacco Nature (Lond.) New Biol. 244, 401–403 (1973)
Maliga, P., Sz.-Breznovits, Á., Márton, L., Joó, F.: Non-Mendelian streptomycin-resistant tobacco mutant with altered chloroplasts and mitochondria. Nature (Lond.) 255, 401–403 (1975)
Márton, L., Maliga, P.: Control of resistance in tobacco cells to 5-bromodeoxyuridine by a simple Mendelian factor. Plant Sci. Letters 5, 77–81 (1975)
Maurer, H.R.: Disc electrophoresis and related techniques of polyacrylamide gel electrophoresis. Berlin-New York: Walter de Gruyter 1971
Meins, F.: Mechanisms underlaying the persistance of tumor autonomy in crown gall disease. In: Tissue culture and plant science (ed. H.E. Street), pp. 233–264. London-New York-San Francisco: Academic Press 1974
Melchers, G. Transformation or habituation to autotrophy and tumor growth and recovery. In: Les cultures de tissues de plantes, pp. 229–234. Paris. Colloq. Inter. C.N.R.S. No. 193, Editions du Centre Nat. Rech. Scient. 1971
Melchers, G., Jockusch, H., v. Sengbusch, P.: A tobacco mutant with a dominant allele for hypersensitivity against some TMV-strains. Phytopath. Z. 55, 86–88 (1966)
Melchers, G., Labib, G.: Somatic hybridisation of plants by fusion of protoplasts. Molec. gen. Genet. 135, 227–294 (1974)
Nagata, T., Takebe, I. Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta (Berl.) 99, 12–20 (1971)
Pestka, S.: Inhibitors of ribosome functions. Ann. Rev. Microbiol. 25, 487–513 (1971)
Pöche, H., Junghahn, I., Geissler, E., Bielke, H.: Cycloheximideresistance in Chinese hamster cells. Molec. gen. Genet. 138, 173–177 (1975)
Rédei, G.P., Acedo, G., Weingarten, H., Kier, L.D.: Has DNA corrected genetically thiamineless mutants of Arabidopsis? In: Cell genetics of higher plants (eds.: D. Dudits, G.L. Farkas and P. Maliga. Budapest: Publishing House of the Hungarian Academy of Sciences 1976 (in the press)
Takebe, I., Otsuki, Y., Aoki, S.: Isolation of tobacco mesophyll cells in intact and active state. Plant and Cell Physiol. 9, 115–124 (1968)
Widholm, J.M.: Selection and characteristics of biochemical mutants of cultured plant cells. In: Tissue culture and plant science (ed.: H.E. Street) pp. 287–299. Oxford: Blackwell 1974
Wilkie, D., Lee, B.K.: Genetic analysis of actidione resistance in Saccharomyces cerevisiae. Genet. Res. (Camb.) 130–138 (1965)
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Experiments with protoplasts were carried out by P.M. and Z.S. in the MPI für Biologie, Abt. Melchers, Tübingen, Bundesrepublik Deutschland
Rights and permissions
About this article
Cite this article
Maliga, P., Lázár, G., Sváb, Z. et al. Transient cycloheximide resistance in a tobacco cell line. Molec. Gen. Genet. 149, 267–271 (1976). https://doi.org/10.1007/BF00268527
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00268527