Skip to main content
SpringerLink
Log in

Vacuolar yield and alteration with aging of suspension-cultured cells from pear fruit

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Vacuoles were isolated from suspension-cultured pear fruit (Pyrus communis L. Passe Crassane) cells of various ages. Yield of vacuoles averaged 17% of protoplasts throughout all the experimental stages. The isolated vacuoles were progressively larger and more likely to stain with neutral red as cells advanced from division to growth and incipient senescence. A relationship between aging and vacuolar or membrane function was implicit in the size and staining characteristics of isolated vacuoles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alibert G, Carrasco A, Boudet M (1982) Biochem Biophys Acta 721:22–29

    Google Scholar 

  • Biale JB, Young RF (1981) In: Friend J and Rhodes MJC (eds) Recent advances in the biochemistry of fruit and vegetables, Acad Press, NY, pp 1–39

    Google Scholar 

  • Bergmeyer HU (1974a) In: Bergmeyer H (ed) Methods of Enzymatic Analysis, vol 3, Verlag Chemie Weinheim-Academic Press, New York, pp 458–459

    Google Scholar 

  • Bergmeyer HU (1974b) In: Bergmeyer H (ed) Methods of Enzymatic Analysis, vol 3, Verlag Chemie Weinheim-Academic Press, New York, pp 501–502

    Google Scholar 

  • Fleischer S, Fleischer B (1967) Methods Enzymol 10:406–433

    Google Scholar 

  • Glund K, Tewes A, Abel S, Leinhos V, Walther R, Reinbothe H (1984) Z. Pflanzenphysiol Bd 113:151–161

    Google Scholar 

  • Nishimura M (1982) Plant Physiol 70:742–744

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    Google Scholar 

  • Nitsh JP, Asahira T, Rossin LME, Nitsch C (1970) Bull Soc Bot Fr 117:479–492

    Google Scholar 

  • Nooden LD (1980) In: Thimann KV (ed), Senescence in Plants, CRC Press, Boca Raton, Florida, pp 219–258

    Google Scholar 

  • Pech JC, Romani RJ (1979) Plant Physiol 64:814–817

    Google Scholar 

  • Puschmann P, Ke D, Romani R (1985) Plant Physiol 79:973–976

    Google Scholar 

  • Romani RJ (1986) In: Bonga J, Durzan D (eds), Cell Culture in Forestry, vol 2, Martinus Nijhoff/W Junk Pub, The Hague, pp 390–404

    Google Scholar 

  • Smith L (1954) In: Glick D (ed) Methods of Biochemical Analysis, vol 2, Interscience Publishers, New York, pp 427–434

    Google Scholar 

  • Thom M, Maretzki A, Komor E (1982) Plant Physiol 69:1315–1319

    Google Scholar 

  • Yamaki S (1984) Plant and Cell Physiol 25:151–166

    Google Scholar 

Download references

Author information

Author notes

  1. Shohei Yamaki

    Present address: Fruit Tree Research Station (Ministry of Agriculture, Forestry and Fisheries), Tsukuba, 305, Ibaraki-pref., Japan

Authors and Affiliations

  1. Department of Pomology, University of California, 95616, Davis, CA, USA

    Shohei Yamaki & Roger Romani

Authors
  1. Shohei Yamaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Roger Romani
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamaki, S., Romani, R. Vacuolar yield and alteration with aging of suspension-cultured cells from pear fruit. Plant Cell Reports 6, 244–247 (1987). https://doi.org/10.1007/BF00268491

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268491

Keywords

Search

Navigation