Summary
Lambda phages carrying the Escherichia coli genes ksgA and pdxA were isolated from secondary site lysogens in araB. 1) The phage genomes were characterized by genetic complementation tests, restriction endonuclease digestion and electron microscopy. 2) A 6.3 kilobasepair (kb) EcoRI restriction fragment carrying both ksgA and pdxA was cloned in a lambda vector; this fragment has proven useful in further characterization of the ksgA gene (Andrésson and Davies, 1980a, b). The ksgA and pdxA genes are about 14 and 12–13 kb, respectively, counterclockwise of the arabinose operon and 1.5 and 2.5–3.5 kb clockwise of folA.
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Andrésson, Ó.S., Davies, J.E.: Genetic organization and restriction enzyme cleavage map of the ksgA-pdxA region of the Escherichia coli chromosome. Mol. Gen. Genet. 179, 211–216 (1980a)
Andrésson, Ó.S., Davies, J.E.: Some properties of the ribosomal RNA methyltransferase encoded by ksgA and the polarity of ksgA transcription. Mol. Gen. Genet. 179, 217–222 (1980b)
Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bacteriol. Rev. 40, 116–167 (1976)
Blattner, F.R., Williams, B.G., Blechl, A.E., Denniston-Thompson, K., Faber, H.E., Furlong, L.-A., Grunwald, D.J., Kiefer, D.O., Moore, D.D., Schumm, J.W., Sheldon, E.L., Smithies, O.: Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science 196, 161–169 (1977)
Boulter, J., Lee, N.: Isolation of specialized transducing bacteriophage lambda carrying genes of the L-arabinose operon of Escherichia coli B/r. J. Bacteriol. 123, 1043–1054 (1975)
Daniels, D.L., deWet, J.R., Blattner, F.R.: A genetic and physical map of bacteriophage DNA. J. Virol., in press (1980)
Davidson, N., Szybalski, W.: Physical and chemical characteristics of lambda DNA. In: The Bacteriophage Lambda (A.D. Hershey, ed.), pp. 45–82. New York: Cold Spring Harbor Laboratory 1971
Davis, R.W., Simon, M., Davidson, N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Methods in enzymology (L. Grossman and K. Moldave, eds.), Vol. XXI, pp. 413–427 New York: Academic Press 1971
Epstein, W., Kim, B.S.: Potassium transport loci in Escherichia coli K-12. J. Bacteriol. 108, 639–644 (1971)
Greenfield, L., Boone, T., Wilcox, G.: DNA sequence of the ara-BAD promoter in Escherichia coli B/r. Proc. Natl. Acad. Sci. USA 75, 4724–4728 (1978)
Hagenbüchle, O., Santer, M., Steitz, J.A., Mans, R.J.: Conservation of the primary structure at the 3′ end of 18S rRNA from eucaryotic cells. Cell 13, 551–563 (1978)
Helser, T.L., Davies, J.E., Dahlberg, J.E.: Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli. Nature New Biol. 233, 12–14 (1971)
Helser, T.L., Davies, J.E., Dahlberg, J.E.: Mechanism of kasugamycin resistance in Escherichia coli. Nature New Biol. 235, 6–9 (1972)
Jaskunas, S.R., Burgess, R.R., Nomura, M.: Identification of a gene for the α-subunit of RNA polymerase at the str-spc region of the Escherichia coli chromosome. Proc. Natl. Acad. Sci. USA 72, 5036–5040 (1975)
Kaplan, D.A., Greenfield, L., Boone, T., Wilcox, G.: Hybrid plasmids containing the araBAD genes of Escherichia coli B/r. Gene 3, 177–189 (1978)
Landy, A., Ross, W.: Viral integration and excision: structure of the lambda att sites. Science 197, 1147–1160 (1977)
Lee, N., Carbon, J.: Nucleotide sequence of the 5′ end of araBAD operon messenger RNA in Escherichia coli B/r. Proc. Natl. Acad. Sci. USA 74, 49–53 (1977)
Legerski, R.J., Hodnett, J.L., Gray, H.B.: Extracellular nucleases of Pseudomonas BAL 31. III. Use of the double-strand deoxyribonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes. Nucleic Acids 5, 1445–1464 (1978)
Lennox, E.S.: Translation of linked genetic characters of the host by bacteriophage Pl. Virology 1, 190–206 (1955)
Lis, J.T., Schleif, R.F.: The isolation and characterization of plaque-forming arabinose transducing phage. J. Mol. Biol. 95, 395–407 (1975)
Miller, J.H.: Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory 1972
Okuyama, A., Watanabe, T., Tanaka, N.: Effects of aminoglycoside antibiotics on initiation of viral RNA-directed protein synthesis. J. Antibiot. 25, 212–218 (1972)
Poldermans, B., Van Buul, C.P.J.J., Van Knippenberg, P.H.: Studies on the function of two adjacent N6, N6-dimethyladenosines near the 3′ end of 16S ribosomal RNA of Escherichia coli. II. The effect of the absence of the methyl groups on initiation of protein biosynthesis. J. Biol. Chem. 254, 9090–9094 (1979)
Postle, K., Reznikoff, W.S.: HindII and HindIII restriction maps of the attϕ80-tonB-trp region of the Escherichia coli genome, and location of the tonB gene. J. Bacteriol. 136, 1165–1173 (1978)
Robinson, L.H., Landy, A.: HindII, HindIII and HpaI restriction fragment maps of bacteriophage λDNA. Gene 2, 1–31 (1977a)
Robinson, L.H., Landy, A.: HindII, HindIII and HpaI restriction fragment maps of the left arm of bacteriophage λ DNA. Gene 2, 33–54 (1977b)
Saltzman, L., Apirion, D.: Binding of erythromycin to the 50S ribosomal subunit is affected by alterations in the 30S ribosomal subunit. Mol. Gen. Genet. 143, 301–306 (1976)
Saltzman, L.A., Brown, M., Apirion, D.: Functional interdependence among ribosomal elements as revealed by genetic analysis. Mol. Gen. Genet. 133, 201–207 (1974)
Sparling, P.F.: Kasugamycin resistance: 30S ribosomal mutation with an unusual location on the Escherchia coli chromosome. Science 167, 56–58 (1970)
Steitz, J.A., Jakes, K.: How ribosomes select initiator regions in mRNA: Base pair formation between the 3′terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli. Proc. Natl. Acad. Sci. USA 72, 4734–4738 (1975)
Tanaka, T., Weisblum, B.: Construction of a colicin El-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J. Bacteriol. 121, 354–362 (1975)
Thorbjarnardóttir, S.H., Magnúsdóttir, R.A., Eggertsson, G., Kagan, S.A., Andrésson, Ó.S.: Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli. Mol. Gen. Genet. 161, 89–98 (1978)
Wu, T.T.: A model for three-point analysis of random general transduction. Genetics 54, 405–440 (1966)
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Andrésson, Ó.S., Davies, J.E. Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA . Molec. Gen. Genet. 179, 201–209 (1980). https://doi.org/10.1007/BF00268464
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DOI: https://doi.org/10.1007/BF00268464