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Isolation of cell cycle mutants of Physarum polycephalum

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Summary

Asynchronous amoebal cultures of temperature-sensitive mutants of Physarum polycephalum were examined cytologically, and two cell cycle mutants were identified. Genetic analysis indicated that each mutant carried a single mutation that was expressed in both amoebal and plasmodial phases. Thus it is possible to isolate cell cycle mutations expressed in plasmodia by initial isolation and analysis of amoebal mutants, a quicker procedure than the alternative of isolating plasmodial mutants directly. The two mutants were studied further by measuring nuclear DNA contents and synthesis of macromolecules. Both mutants gave results consistent with a block in nuclear division.

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Author notes

  1. Timothy G. Burland

    Present address: Institute of Medical Biology, University of Tromsø, P.O. Box 977, N-9001, Tromsø, Norway

Authors and Affiliations

  1. Genetics Dept., University of Leicester, LE1 7RH, Leicester, England

    Timothy G. Burland & Jennifer Dee

Authors
  1. Timothy G. Burland
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  2. Jennifer Dee
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Communicated by C. Auerbach

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Burland, T.G., Dee, J. Isolation of cell cycle mutants of Physarum polycephalum . Molec. Gen. Genet. 179, 43–48 (1980). https://doi.org/10.1007/BF00268444

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  • DOI: https://doi.org/10.1007/BF00268444

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