Summary
sbcA, a gene defined by mutations which restore recombination proficiency to RecB and RecC mutants, has been located at minute 29 of the E. coli genetic map. Two independent sbcA mutations have been mapped in this location.
In an Hfr recB - sbcA - rac? x F- recB - sbcA + rac +cross, recombinant progeny inheriting the donor his + or trp + allele also inherited sbcA - at a high frequency. Reciprocal crosses using sbcA + Hfrs and recB - sbcA -females located sbcA in that region of the chromosome represented between his and trp on the genetic map. This position was confirmed in conjugation experiments by the time of entry of sbcA + (as an unselected marker) using two Hfrs transferring trp + early from opposite directions. These results showed that sbcA is close to trp and clockwise from it between 27–29 minutes. Since we detected no cotransduction (less than 0.2%) of sbcA and trp, we place sbcA at minute 29.
The observation that merodiploids with the recB - sbcA -/F′ sbcA + genotype were as sensitive to UV as haploid strains with the recB - sbcA +genotype indicated that sbcA +is dominant to sbcA -.
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Communicated by W. Maas and B. A. Bridges
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Lloyd, R.G., Barbour, S.D. The genetic location of the sbcA gene of Escherichia coli . Molec. Gen. Genet. 134, 157–171 (1974). https://doi.org/10.1007/BF00268417
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DOI: https://doi.org/10.1007/BF00268417