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The induction of mitotic gene conversion by chemical and physical mutagens as a function of culture age in the yeast, Saccharomyces cerevisiae

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Summary

Cultures of yeast progressing from the exponential to the stationary phase of growth show increased resistance to the lethal effects of the chemical mutagens nitrous acid, ethyl methane sulphonate and mitomycin C and increased sensitivity to the lethal effects of UV light. Induced mitotic intragenic recombination produced by gene conversion also shows variation in its response to the growth phase after mutagen treatment. Higher frequencies of recombination per surviving cell were found after nitrous acid and ethyl methane sulphonate treatment of stationary phase cells wherease identical frequencies were produced by UV and mitomycin C treatment in both growth phases.

The results were consistent with the hypothesis that the more nitrous acid and ethyl methane sulphonate resistant stationary phase cells were more active in postreplication repair. The sensitivity of exponential phase cells to nitrous acid and ethyl methane sulphonate may result from both increased mutagen uptake and reduced postreplication repair activity. In contrast, irrespective of growth phase all cells surviving UV and mitomycin C treatment appear to have undergone identical levels of post-replication repair.

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References

  • Cole, R.S.: Repair of DNA containing interstrand crosslinks. Proc. nat. Acad. Sci. (Wash.) 70, 1064–1068 (1973)

    Google Scholar 

  • Davies, P.J., Evans, W.E., Parry, J.M.: Mitotic recombination induced by chemical and physical agents in the yeast Saccharomyces cerevisiae. Mutation Res. 29, 301–314 (1975)

    Google Scholar 

  • Deutch, C.E., Parry, J.M.: Sphaeroplast formation in yeast during the transition from exponential phase to stationary phase. J. gen. Microbiol. 80, 259–268 (1974)

    Google Scholar 

  • Fabre, F.: UV-sensitivity of the wild-type and different UVS mutants of Schizosaccharomyces pombe: Influence of growth stages and DNA content of the cells. Mutation Res. 10, 415–426 (1970)

    Google Scholar 

  • Fink, G.R.: A cluster of genes controlling three enzymes in histidine biosynthesis in Saccharomyces cerevisiae. Genetics 53, 445–459 (1966)

    Google Scholar 

  • Holliday, R: Altered recombination frequency in radiation sensitive strains of Ustilago maydis. Mutation Res. 4, 275–288 (1967)

    Google Scholar 

  • Holliday, R.: Biochemical measure of the time and frequency of radiation induced allelic recombination in Ustilago. Nature (Lond.) 232, 233–236 (1971)

    Google Scholar 

  • Holliday, R.: Further evidence for an inducible recombination repair system in Ustilago maydis. Mutation Res. 29, 149–153 (1975)

    Google Scholar 

  • Howard-Flanders, P., Lin, P.F.: Genetic recombination induced by DNA cross-links in repressed phage Lambda. Genetics 73, Suppl. 85–90 (1973)

    Google Scholar 

  • Howell-Saxton, E., Smith, D.C., Zamenhoff, P.J., Zamenhoff, S.: Effects of growth phase and repair capacity on rejoining of ethyl methane sulphonate-induced DNA breaks in Escherichia coli. Mutation Res. 24, 227–237 (1974)

    Google Scholar 

  • Parry, J.M., Davies, P.J., Evans, W.E.: The effects of “cell age” upon the lethal effects of physical and chemical mutagens in the yeast, Saccharomyces cerevisiae. The effects of “cell age” upon the lethal effects of physical and chemical mutagens in the yeast. Saccaromyces cerevisiae. Molec. gen. Genet. 146, 27–35 (1976)

    Google Scholar 

  • Parry, J.M., Parry, E.M., Davies, P.J., Evans, W.E.: Cell division and radiation sensitivity in yeast. In press (1976)

  • Tyrell, R.M., Moss, S.H., Davies, D.J.G.: The variation in UV sensitivity of our K-12 strains of Escherichia coli as a function of their stage of growth. Mutation Res. 16, 1–12 (1972)

    Google Scholar 

  • Yos, H.T., Chalett, R.S., Finerty, J.P.: Induction of mitotic recombination in Saccharomyces cerevisiae by ethyl methane sulphonate. Nature (Lond.) 215, 660–661 (1967)

    Google Scholar 

  • Zimmermann, F.K., Schwaier, R.: Induction of mitotic gene conversion with nitrous acid, 1-methyl-3-nitro-1-nitroguanidine and other alkylating agents in Saccharomyces cerevisiae. Molec. gen. Genet. 100, 63–76 (1967)

    Google Scholar 

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Author notes

  1. Peter J. Davies

    Present address: Institute für Genetik der Universität zu Köln, D-5000, Köln 41, Federal Republic of Germany

Authors and Affiliations

  1. Department of Genetics, University College of Swansea, SA2 8PP, Swansea, U.K.

    Peter J. Davies & James M. Parry

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  1. Peter J. Davies
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  2. James M. Parry
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Communicited by F. Kaudewitz

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Davies, P.J., Parry, J.M. The induction of mitotic gene conversion by chemical and physical mutagens as a function of culture age in the yeast, Saccharomyces cerevisiae . Molec. Gen. Genet. 148, 165–170 (1976). https://doi.org/10.1007/BF00268381

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  • DOI: https://doi.org/10.1007/BF00268381

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