Summary
The fate of the donor DNA after conjugation in Escherichia coli was studied through crosses with a Hfr lacZ5 donor and several F- lacZ22 recipients. The fate of the donor allele was studied by assaying the β-galactosidase activity formed by complementation between the lacZ5 allele and the lacZ22 allele. We used continuous cultures of the recipient in order to be able to study the fate of the donor DNA during many generations under constant physiological conditions. We could show that the donor DNA allele is inactivated in Rec+, recA171 and recB21 recipient cells. The inactivation rate depends on the nature of the recipient, Rec+ or recombination deficient, and especially in the case of the recombination deficient mutants on the growth rate of the recipient.
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Bergmans, H.E.N., Hoekstra, W.P.M. The fate of the donor DNA after conjugation in Escherichia coli K12: Influence of the growth rate of the recipient. Molec. Gen. Genet. 158, 179–183 (1977). https://doi.org/10.1007/BF00268311
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DOI: https://doi.org/10.1007/BF00268311