Summary
A thermosensitive mutant (PD ts11 ) of Chlamydomonas reinhardi producing the neutral phosphatase at 25°C but not at 35°C was investigated to understand the role of the corresponding gene in the formation of the neutral phosphatase.
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1.
The mutation only affects the formation of the neutral phosphatase and not that of the alkaline phosphatases.
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2.
It is recessive to its wild allele.
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3.
The production of the neutral phosphatase in Pi-starved cells is dependent upon protein synthesis as suggested by the fact that it rapidly stops when the cells are exposed to cycloheximide.
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4.
As shown by immunodiffusion experiments, PD ts11 cells grown at 35°C do not contain the antigen related to the neutral phosphatase which is present in cells grown at 25°C.
These results suggest that PD ts11 is a regulatory gene exerting a positive control on the synthesis of the neutral phosphatase.
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Communicated by F. Gros
Chercheur qualifié du Fonds National Belge de la Recherche Scientifique
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Loppes, R. Regulation of the neutral phosphatase in Chlamydomonas reinhardi: Study of a thermosensitive mutant. Molec. Gen. Genet. 158, 165–169 (1977). https://doi.org/10.1007/BF00268309
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DOI: https://doi.org/10.1007/BF00268309