Summary
Repair replication of DNA has been studied in first instar larvae and cultured cells of meiotic-9 (mei-9) mutants in Drosophila melanogaster. Results obtained with both experimental systems show that the mei-9 mutants are defective in this form of repair after UV and X-ray treatment. This defect is correlated with the observation that male larvae carrying alleles of this locus are hypersensitive to killing by UV and X-rays. These findings strengthen the suggestion derived from genetic data that the normal mei-9 + function is involved in the exchange process of meiotic recombination rather than in a precondition to exchange (Carpenter and Sandler, 1974).
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Abbreviations
- FdUrd:
-
5-fluorodeoxyuridine
- BrdUrd:
-
5-bromodeoxyuridine
- 3H-dThd:
-
thymidine methyl-3H
- SSC:
-
0.15 M sodium chloride-0.015 M sodium citrate
- UV:
-
ultraviolet radiation-predominantly 254 nm
References
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Communicated by B.A. Bridges
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Nguyen, T.D., Boyd, J.B. The meiotic-9 (mei-9) mutants of Drosophila melanogaster are deficient in repair replication of DNA. Molec. Gen. Genet. 158, 141–147 (1977). https://doi.org/10.1007/BF00268306
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DOI: https://doi.org/10.1007/BF00268306