Summary
Sodium nalidixate inhibited the cell growth and division of several respiratory competent strains of Saccharomyces cerevisiae. A number of cytoplasmic petite strains (both spontaneous and induced by ethidium bromide) were shown to be more resistant to sodium nalidixate than the wild-type strains from which they were derived. There was considerable variation in sensitivity of different petites derived from the same wild-type. Usually petite strains which were induced by ethidium bromide were more resistant than spontaneously arising petites. The susceptibility of a wild-type strain to nalidixate was found to be least when the mitochondrial respiratory system was maximally repressed. It was also noted that sodium nalidixate (100 μg/ml) induced petite mutants.
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Communicated by F. Kaudewitz
Dr. Carnevali is a Senior Research Worker of the Centro di Studio per gli Acidi Nucleici of the National Research Council of Rome and is on leave of absence at the above address
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Carnevali, F., Sarcoe, L.E. & Whittaker, P.A. Differential effects of nalidixate on the cell growth of respiratory competent strains and cytoplasmic petite mutants of Saccharomyces cerevisiae . Molec. Gen. Genet. 146, 95–100 (1976). https://doi.org/10.1007/BF00267988
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DOI: https://doi.org/10.1007/BF00267988