Summary
In certain HfrxF− recB − crosses recombinant progeny were examined for their SbcA and Rac phenotypes. Recombinants which inherited either his + or trp + from the donor in an Hfr recB21 sbcA8xF− recB21 Rac−SbcA+ cross acquired the RecB+ phenotype in most instances (presumably by inheriting the sbcA8 allele). Several independent Rec+ (sbcA8) recombinants from this cross were converted back to the Rec− (sbcA +) phenotype by mating with a Rac+ SbcA+ Hfr. Ten out of 14 of these Rec− recombinants retained the Rac− phenotype of the original parent. It was concluded that these results were inconsistent with the hypothesis that sbcA is a gene carried by a “Rac prophage”.
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Communicated by B. A. Bridges and W. Maas
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Lloyd, R.G. The segregation of the SbcA and Rac phenotypes in an Escherichia coli recB − mutant. Molec. Gen. Genet. 134, 249–259 (1974). https://doi.org/10.1007/BF00267719
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DOI: https://doi.org/10.1007/BF00267719