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Mapping of gshA, a gene for the biosynthesis of glutathione in Eschericha coli K12

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Summary

A mutation affecting the activity of the first enzyme in glutathione biosynthesis in E. coli K 12 has been mapped. The mutant allele called gshA is located by transduction in the pheA-nalB segment. The linkages with pheA and tyrA provide a convenient method for transfer of gshA to other strains and so introduce a very low level of non-protein sulfhydryl groups into the E. coli cells.

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Authors and Affiliations

  1. Biochemical and Biophysical Laboratory, Delft University of Technology, Delft, (The Netherlands)

    P. Apontoweil & W. Berends

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  1. P. Apontoweil
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  2. W. Berends
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Additional information

Communicated by F. Gros

This paper will be used in a thesis to be submitted by P. Apontoweil to the Delft University of Technology in partial fulfilment of the requirements for the Ph.D. degree.

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Apontoweil, P., Berends, W. Mapping of gshA, a gene for the biosynthesis of glutathione in Eschericha coli K12. Molec. Gen. Genet. 141, 91–95 (1975). https://doi.org/10.1007/BF00267676

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  • DOI: https://doi.org/10.1007/BF00267676

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