Summary
When supplemented with Escherichia coli stringgent factor, 80S ribosomes from various sources failed to support guanosine tetra- and pentaphosphate ((p)ppGpp) synthesis. In contrast, ribosomal proteins from 80S, 60S or 40S particles (mouse embryos, rabbit reticulocytes) crossreacted with the E. coli stringent factor. Significant stimulation of (p)ppGpp synthesis was achieved proteins/ml. These observations may provide additional criteria to detect homologies between eukaryotic and prokaryotic ribosomal proteins.
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Martini, O., Richter, D. Eukaryotic ribosomal proteins stimulate Escherichia coli stringent factor to synthesize guanosine 5′-diphosphate, 3′-diphosphate (ppGpp) and guanosine 5′-triphosphate, 3′-diphosphate (pppGpp). Molec. Gen. Genet. 166, 291–297 (1978). https://doi.org/10.1007/BF00267621
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DOI: https://doi.org/10.1007/BF00267621