Summary
Mutants of Pseudomonas aeruginosa PAO deficient in their utilization of DL-valine have been found to have lost their capacity to utilize DL-alanine and L-proline. Use of conjugal and transductional mediated gene transfers have established the chromosomal location of this gene and also its pleotropic function in the induction of the D-amino acid oxidase, involved in the oxidative utilization of DL-valine, DL-alanine and L-proline. These point mutations are clustered in a single locus designated as Val D and mapped around the 19th minute on the chromosome.
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Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K12. Bact. Rev. 40, 116–167 (1976)
Bater, A.J., Vanables, W.A., Thomas, S.: Allohydroxy-D-proline dehydrogenase: An inducible membrane-bound enzyme in Pseudomonas aeruginosa PA01. Arch. Microbiol. 112, 287–289 (1977)
Clarke, P.H., Ornston, L.N.: Metabolic pathways and regulation. In: Genetics and biochemistry of Pseudomonas (P.H. Clarke and M.H. Richmond, eds.), pp. 191–340. London: J. Wiley & Sons 1975
Conrad, R.S., Massey, L.K., Sokatch, J.R.: D- and L-isoleucine metabolism and regulation of their pathways in Pseudomonas putida. J. Bact. 118, 103–111 (1974)
Day, M., Potts, J.R., Clarke, P.H.: Location of genes for the utilization of acetamide, histidine and proline on the chromosome of Pseudomonas aeruginosa. Genet. Res. 25, 71–78 (1975)
Gryder, R.M., Adams, E.: Inducible degradation of hydroxyproline in Pseudomonas putida: Pathway regulation and hydroxyproline uptake. J. Bact. 97, 292–306 (1969)
Haas, D., Holloway, B.W.: R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Molec. gen. Genet. 144, 243–251 (1976)
Haas, D., Holloway, B.W., Schambock, A., Lesinger, T.: The genetic organisation of arginine biosynthesis in Pseudomonas aeruginosa. Molec. gen. Genet. 154, 7–22 (1977)
Hader, R., Slonim, A., Kuhn, J.: Role of D-tryptophan oxidase in D-tryptophan utilization by Escherichia coli. J. Bact. 125, 1096–1104 (1976)
Isaac, J.H., Holloway, B.W.: Control of pyrimidine biosynthesis in Pseudomonas aeruginosa. J. Bact. 96, 1732–1941 (1968)
Jayaraman, K., Radhakrishnan, A.N.: Bacterial metabolism of hydroxyproline: Part I—Metabolism of L-allohydroxyproline by Pseudomonas. Indian J. Biochem. 2, 145–152 (1965a)
Jayaraman, K., Radhakrishnan, A.N.: Bacterial metabolism of hydroxyproline: Part II—Metabolism of L-hydroxyproline in Achromobacter. Indian J. Biochem. 2, 153–158 (1965b)
Leidigh, B.J., Wheelis, M.L.: The clustering on the Pseudomonas putida chromosome of genes specifying dissimiatory functions. J. Molec. Evolution 2, 235–242 (1973)
Lowry, O.H., Resebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951a)
Manoharan, H.T., Jayaraman, K.: Evidence for the presence of two D-amino acid oxidases in Pseudomonas aeruginosa PAO Proc. Indian Acad. Sci. 87 B(1), 25–29 (1978)
Marshall, V.P., Sokatch, J.R.: Oxidation of D-amino acids by a particulate enzyme from Pseudomonas aeruginosa. J. Bact. 95, 1419–1424 (1968)
Marshall, V.P., Sokatch, J.R.: Regulation of valine catabolism in Pseudomonas putida. J. Bact. 110, 1073–1081 (1972)
Martin, R.R., Marshall, V.P., Sokatch, J.R., Unger, L.: Common enzymes of branched-chain amino acid catabolism in Pseudomonas putida. J. Bact. 115, 198–204 (1973)
Massey, K.L., Conrad, R.S., Sokatch, J.R.: Regulation of leucine catabolism in Pseudomonas putida. J. Bact. 118, 112–120 (1974)
Mee, B.J., Lee, B.T.O.: A map order for his I, one of the genetic regions controlling histidine biosynthesis in Pseudomonas aeruginosa, using the transducing phage F116. Genetics 62, 687–696 (1968)
Olsen, R.H., Hansen, J.: Evolution and utility of a Pseudomonas aeruginosa, drug resistance factor. J. Bact. 125 837–844 (1976)
Pioli, D., Vanables, W.A., Frankline, F.C.H.: D-alanine dehydrogenase: its role in the utilization of alanine isomers as growth substrate by Pseudomonas aeruginosa PAO. Arch. Microbiol. 110, 287–293 (1976)
Radhakrishnan, A.N., Meister, A.: Conversion of hydroxyproline to pyrole-2-carboxylic acid. J. biol. Chem. 226, 559–571 (1957)
Stanisich, V.A., Holloway, B.W.: A mutant sex factor of Pseudomonas aeruginosa. Genet. Res. 19, 91–108 (1972)
Wellner, D., Scannone, H.: Oxidation of L-proline and L-3,4-dehydroproline by D-amino acid oxidase. Biochemistry 3, 1946–1949 (1964)
Wheelis, M.L., Stanier, R.Y.: The genetic control of dissimiliatory pathways in Pseudomonas putida. Genetics 66, 245–266 (1970)
Wild, I., Walczak, W., Krajewska-Gryniewicz, K., Klopotowski, T.: D-amino acid dehydrogenase: The enzyme of the first step of D-histidine and D-methionine recemization in Salmonella typhimurium. Molec. gen. Genet. 128, 131–146 (1974)
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Manoharan, T.H., Jayaraman, K. Mapping of the locus involved in the catabolic oxidation of D-amino acids in Pseudomonas aeruginosa PAO. Molec. Gen. Genet. 164, 51–56 (1978). https://doi.org/10.1007/BF00267598
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DOI: https://doi.org/10.1007/BF00267598