Summary
A single recessive nuclear gene mutation has been isolated from strain 123.1 C of Saccharomyces cerevisiae which is conditionally deficient in mitochondrial DNA metabolism and has been termed tpi. Growth of this mutant strain in media containing galactose at 36°C causes a reduction of mitochondrial DNA synthesis as analyzed by incorporation of radioactive adenine into the mitochondrial DNA. These cells continue to grow and divide producing petite cells which are neutral and have been found to lack mitochondrial DNA as measured by radioactive incorporation of 3H-adenine into the mitochondrial DNA in the presence of cycloheximide at the permissive temperature. The rate of mitochondrial DNA synthesis of the mutant strain grown at the restrictive temperature in dextrose or glycerol containing media was found to be greatly reduced following two hours of exposure to the restrictive temperature. In addition, the action of this mutant gene has been found to be independent of the respiratory capacity of the mutant strain.
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References
Criddle, R.S., Schatz, G.: Promitochondria of anaerobically grown yeast. I. Isolation and biochemical properties. Biochemistry 8, 322–334 (1969)
Grossman, L.I., Goldring, E.S., Marmur, J.: Preferential synthesis of yeast mitochondrial DNA in the absence of protein synthesis. J. molec. Biol. 46, 369–376 (1969)
Hereford, L.M., Hartwell, L.H.: Role of protein synthesis in the replication of yeast DNA. Nature New Biol. 244, 129–131 (1973)
Hollenberg, C.P., Borst, P.: Conditions that prevent ρ− induction by ethidium bromide. Biochem. biophys. Res. Commu. 45, 1250–1254 (1971)
Newlon, C.S., Fangman, W.L.: Mitochondrial DNA synthesis in cell cycle mutants of Saccharomyces cerevisiae. Cell 5, 423–428 (1975)
Petes, T., Fangman, W.L.: Preferential synthesis of yeast mitochondrial DNA in α-factor arrested cells. Biochem. biophys. Res. Commun. 55, 603–609 (1973)
Pinto, M., Guerineau, M., Paoletti, C.: Ethidium bromide mutagenesis in yeast: Protection by anaerobiosis. Mut. Res. 30, 219–228 (1975)
Rubin, B.Y., Blamire, J.: Regulation of yeast mitochondrial DNA synthesis I. Analysis of a mutant conditionally deficient in mitochondrial DNA metabolism. Molec. gen. Genet. 156, 41–47 (1977)
Vidova, M., Kovac, L.: Nalidixic acid prevents the induction of yeast cytoplasmic respiration-deficient mutants by intercalating drugs. FEBS Lett. 22, 347–351 (1972)
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Communicated by F. Kaudewitz
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Rubin, B.Y., Blamire, J. Regulation of yeast mitochondrial DNA synthesis. Molec. Gen. Genet. 169, 41–47 (1979). https://doi.org/10.1007/BF00267543
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DOI: https://doi.org/10.1007/BF00267543