Summary
Genetic studies were made on the genes determining the ability to ferment starch (STA) in five strains of S. diastaticus different in origins, strain IFO 1046, IFO 1015, Y 8, Y 13 and C1372. With the heterothallic strain IFO 1046, the genes STA1 and STA2 were separated by single spore cultures and crosses to S. cerevisiae. Strain Y 8 did not sporulate, but the progeny having gene STA3 was isolated from the hybrid between the respiratory deficient mutant of strain Y 8 and S. cerevisiae obtained by the minimal plate mating technique. Strain IFO 1015 was the homothallic strain. The progeny having gene STA4 was isolated from the hybrid between IFO 1015 and S. cerevisiae. Strain Y 13 did not sporulate, but the progeny having gene STA5 was isolated according to the same technique as applied to strain Y 8. Haploid strain C1372 retained the gene STA6. The genes STA1 and STA4, and STA3, STA5 and STA6 were shown to be not separable by tetrad analysis.
These results demonstrate the existence in S. diastaticus of at least 3 polymorphic genes for starch fermentation; STA1 (STA4), STA3 (STA5 and STA6), and STA2, and these STA genes are located on different linkage groups.
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Communicated by F. Kaudewitz
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Tamaki, H. Genetic studies of ability to ferment starch in Saccharomyces: Gene polymorphism. Molec. Gen. Genet. 164, 205–209 (1978). https://doi.org/10.1007/BF00267385
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DOI: https://doi.org/10.1007/BF00267385