Summary
Repressed lambda chromosomes that possess a duplication of the cohesive end site (cos site) are matured during lytic growth of ϕ80. This result is in contrast to repressed non-duplication lambda chromosomes that are not matured by ϕ80. DNA molecules matured by ϕ80 are unreplicated and lack the duplication: both cos sites are cleaved. These results indicate that in normal lambda development, mature, unit-length chromosomes are generated from a multichromosomal length of lambda DNA by cleavage of two cos sites.
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Communicated by F. Stahl
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Feiss, M., Margulies, T. On maturation of the bacteriophage lambda chromosome. Molec. Gen. Genet. 127, 285–295 (1973). https://doi.org/10.1007/BF00267099
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DOI: https://doi.org/10.1007/BF00267099