Summary
From Citrobacter freundii mutants have been isolated, with deletions extending chl genes. 53% of these mutants mapped in the gal-bio region of the chromosome. Genetic mapping by three methods—deletion analysis, linkage analysis in crosses with C. freundii Hfr donors and complementation with Escherichia coli F′ factors—establishes the gene order: chl H-gal-chl D-hut-bio-uvr B-chl A-chl I-chl E In an other segment a gene order ilv-chl-pdx was found. Furthermore chl loci were found adjacent to 7 different chromosomal markers.
C. freundii can form nitrate reductase A, thiosulfate reductase, tetrathionate reductase and formic dehydrogenase. These enzymes are not formed in most of the chlorate-resistant mutants. In some of these mutants the enzyme activities can be restored by complementation with F′ factors of E. coli.
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de Graaff, J., Barendsen, W. & Stouthamer, A.H. Mapping of chlorate-resistant mutants of Citrobacter freundii by deletion and complementation analysis. Molec. Gen. Genet. 121, 259–269 (1973). https://doi.org/10.1007/BF00267053
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DOI: https://doi.org/10.1007/BF00267053