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Genetic fine structure and function of mutants at the ilv1-gene locus of Saccharomyces cerevisiae

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Summary

27 point-mutations at the ilv1 locus of Saccharomyces cerevisiae were subjected to genetical analysis, including fine-structure mapping, interallelic complementation and selection of nonsense supersuppressors. The following information was obtained:

  1. 1.

    A genetic map of the locus was established on the basis of mitotic gene conversion induced with gamma rays. It has a length of about 16 units and fails to show any expansion, contrary to what is generally observed in fungal intragenic maps based on meiotic allelic recombination.

  2. 2.

    A circular complementation map is obtained, the shape of which strongly depends on temperature.

  3. 3.

    Non-complementing alleles are distributed over the entire locus, thus showing that it consists of one cistron.

  4. 4.

    Several complementing and temperature sensitive mutant alleles were found to be suppressible by nonsense-suppressors. These alleles were clustered at one end of the map, thus indicating the direction of translation in respect to the gene. The sense of translation relative to the entire chromosome could be derived because the sequence of two mutant alleles included in the present study had been previously determined in relation to outside markers by Kakar (1963a).

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Author notes

  1. P. Thuriaux & M. Minet

    Present address: Institut für Allgemeine Mikrobiologie, Altenbergrain 21, Bern, Switzerland

  2. A. M. A. ten Berge

    Present address: Van't Hoff Laboratorium, Rijksuniversiteit, Sterrenbos 19, Utrecht, The Netherlands

  3. F. K. Zimmermann

    Present address: Department of Biology, Brooklyn College, 11210, Brooklyn, N.Y.

Authors and Affiliations

  1. Forstbotanisches Institut der Universität Freiburg i. Br., Freiburg, Germany

    P. Thuriaux, M. Minet, A. M. A. ten Berge & F. K. Zimmermann

  2. Department of Biology, Brooklyn College of the City University of New York, 11210, Brooklyn, New York, USA

    F. K. Zimmermann

Authors
  1. P. Thuriaux
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  2. M. Minet
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  3. A. M. A. ten Berge
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  4. F. K. Zimmermann
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Communicated by F. Kaudewitz

This article is dedicated to the wine farmers and cellarmen of the Freiburg area whose delicious products make it a great culinary experience to live there.

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Thuriaux, P., Minet, M., ten Berge, A.M.A. et al. Genetic fine structure and function of mutants at the ilv1-gene locus of Saccharomyces cerevisiae . Molec. Gen. Genet. 112, 60–72 (1971). https://doi.org/10.1007/BF00266933

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  • DOI: https://doi.org/10.1007/BF00266933

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