Summary
The decrease in the solubility of fructose- 1,6-diphosphate-aldolase (fructose-1, 6-diphosphate-D-glyceraldehyde-3-phosphate lyase, E.C.4.1.2.13) brought about by the addition of polyvinyl alcohol to the incubation medium was tested observing histochemical conditions. After 100 minutes of incubation in an aqueous medium less than 10% of the original enzymic activity was demostrated in sections of rat cardiac muscle and kidney. After polyvinyl alcohol was added to the incubation medium the enzymic activity in the sections (measured over the same period) was 80% in rat cardiac muscle and 30–40% in kidney sections. The importance of these findings for the histochemical demonstration of aldolase is shown in the rat cardiac muscle. The organ dependend differences in the solubility may find an explanation in the presence of various iso-enzymes in the two organs under investigation.
Zusammenfassung
Die Verminderung der Löslichkeit der Fructose-1,6-diphosphat-Aldolase (Fructose-1,6-diphosphat-D-Glycerinaldehyd-3-phosphat-Lyase, E.C. 4.1.2.13) durch Zusatz von Polyvinylalkohol zum Inkubationsmedium wurde unter Einhaltung histochemischer Bedingungen überprüft. Bei der Inkubation in wäßrigem Medium sind nach 100 min in einem Schnitt des Herzmuskels und der Niere der Ratte weniger als 10% der ursprünglichen Enzymaktivität nachweisbar. Nach Zusatz von Polyvinylalkohol zum Inkubationsmedium beträgt die im Schnitt verbleibende Enzymaktivität nach gleicher Inkubationsdauer für den Rattenherzmuskel etwa 80%, für die Niere 30–40%. Die Bedeutung dieser Befunde für den histochemischen Nachweis der Aldolase wird am Beispiel des Rattenherzmuskels gezeigt. Als Ursache der organbedingten Unterschiede im Löslichkeitsverhalten wird die Anwesenheit verschiedener Isoenzyme in beiden Organen in Betracht gezogen.
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Arnold, F., Kunze, K.D. & Grossmann, H. Über den Einfluß der Gelinkubation mit Polyvinylalkohol auf die Löslichkeit der Fructose-1,6-diphosphat-Aldolase. Histochemie 13, 196–202 (1968). https://doi.org/10.1007/BF00266581
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DOI: https://doi.org/10.1007/BF00266581