Summary
A quantitative histochemical and biochemical study has been made of the loss of pyridine nucleotide-linked dehydrogenases from frozen histological sections of rat liver. Glucose-6-phosphate, 6-phosphogluconate and lactate dehydrogenases were lost rapidly from the sections during incubation in the histochemical medium, but β-OH-butyrate dehydrogenase was lost at a much slower rate. It was shown that a dehydrogenase reaction can occur in a section lacking that particular dehydrogenase if the section is incubated in the presence of another containing the dehydrogenase. The validity of the tetrazolium reaction for demonstrating pyridine-nucleotide-linked dehydrogenases is considered in the light of these results.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adamstone, F.B., and A.B. Taylor: The rapid preparation of frozen tissue sections. Stain Technol. 23, 109–116 (1948).
Bitensky, L., J. Chayen, and M. McCabe: Ann. Rep. roy. Coll. Surg. Engl., 72 (1962).
Blanchaer, M.C., M. Van Wijke, and D. Mozersky: The oxidation of lactate and α-glycerophosphate by red and white skeletal muscle. I. Quantitative studies. J. Histochem. Cytochem. 11, 500–504 (1963).
Chayen, J., G.J. Cunningham, P.B. Gahan, and A.A. Silcox: Newer methods in cytology. Bull. Res. Counc. Israel D 8, 273–284 (1960).
: Lifelike preservation of cytoplasmic detail in plant cells. Nature (Lond.) 186, 1068–1069 (1960).
Cunningham, G.J., L. Bitensky, J. Chayen, and A.A. Silcox: The preservation of cytological and histochemical detail by a controlled temperature freezing and sectioning technique. Ann. Histochim. 7, 433–435 (1962).
Duve, C. De, R. Wattiaux, and P. Baudhuin: Distribution of enzymes between subcellular fractions in animal tissues. Advanc. Enzymol. 24, 291–358 (1962).
Fahimi, H.D., and C.R. Amarasingham: Cytochemical localisation of lactic dehydrogenase in white skeletal muscle. J. Cell Biol. 22, 29–48 (1964).
Friede, R.L., L.A. Fleming, and M. Knoller: A quantitative appraisal of enzyme histochemical methods in brain tissue. J. Histochem. Cytochem. 11, 232–245 (1963).
Gahan, P.B.: Histochemical evidence for the presence of lysosome-like particles in root meristem cells of Vicia faba. J. exp. Bot. 16, 350–355 (1965).
M. Kalina: The validity of using neotetrazolium for studying labile, NADP-linked dehydrogenases in histological sections; a quantitative study. Biochem. J. (in the press).
Hulme, A.C., J.D. Jones, and L.S.C. Wooltorton: Mitochondrial preparations rom flowers. Nature (Lond.) 201, 795–797 (1964).
Jones, G.R.N.: Quantitative histochemistry; design and use of a simple microcell for standardised incubation on the slide. Stain Technol. 39, 155–162 (1964).
—, A.J. Maple, E.K. Aves, J. Chayen, and G.J. Cunningham: Quantitative histochemistry of succinic dehydrogenase in tissue sections. Nature (Lond.) 197, 568–570 (1963).
Lubkin, V., and M. Carsten: Elimination of dehydration in histological techniques. Science 95, 633–634 (1942).
: Dehydration in histologic embedding eliminated by the use of water-soluble synthetic plastic. Arch. Path. 38, 229–232 (1944).
McCabe, M., and J. Chayen: Ann. Rep. roy. Coll. Surg. Engl. 81 (1963).
Munch-Peterson, A.: In: Methods in enzymology, vol. 1, p. 323 (ed. S.P. Colowick and N.O. Kaplan). New York: Academic Press 1955.
Neilands, J.B.: In: Methods in enzymology, vol. 1, p. 449 (ed. S.P. Colowick and N.O. Kaplan). New York: Academic Press 1955.
Pearse, A.G.E.: Histochemistry, 2nd ed. London: Churchill 1960.
Scarpelli, D.G., and G. Frajola: A biochemical study of formolcalcium fixation of various oxidative enzymes. J. Histochem. Cytochem. 9, 603–604 (1961).
Sloane-Stanley, G.H., and G.R.N. Jones: Simple methods for the determination of very small amounts of nitrogen in organic matter. Biochem. J. 86, 16 p (1963).
Thornburg, W., and P.E. Mengers: An analysis of frozen section techniques: 1. Sectioning of fresh-frozen tissue. J. Histochem. Cytochem. 5, 47–52 (1957).
Williamson, B.H., J. Mellanby, and H.A. Krebs: Enzymic determination of D(−)-β-hydroxybutyric acid and acetoacetic acid in blood. Biochem. J. 82, 90–96 (1962).
Wolman, M.: Problems of fixation in cytology, histology and histochemistry. Int. Rev. Cytol. 4, 79–102 (1955).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kalina, M., Gahan, P.B. A quantitative study of the validity of the histochemical demonstration for pyridine nucleotide-linked dehydrogenases. Histochemie 5, 430–436 (1965). https://doi.org/10.1007/BF00306294
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00306294