Summary
Genetic control of the major zein polypeptides in maize (Zea mays L.) was studied by isoelectric focusing (IEF) in agarose. Linkage relationships were determined by making a number of crosses, then determining the expression of zein polypeptides in backcross seeds. Chromosome linkages were determined by using the markers sugary-1 (for chromosome 4), yellow-8, and a waxy 7–9 translocation (for chromosome 7). Nine zeins were in one linkage group on chromosome 4, six in another linkage group on chromosome 4, and four zeins were in one linkage group on chromosome 7. Some IEF single bands consisted of at least two polypeptides, which were detected by subsequent sodium dodecyl sulfate polyacrylamide gel electrophoresis, by aberrant ratios in backcrosses, or by differing recombination percentages. One zein occurred only in homozygous sugary-1 seeds. Three sets of closely-linked zeins were noted that occurred together almost exclusively in certain inbreds.
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References
Bailey NTJ (1961) Introduction to the mathematical theory of linkage. Oxford University Press, Oxford
Binelli G, Soave C, Ottaviano E (1984) Location and differential expression of a zein gene in maize. Plant Sci Let 33:259–265
Esen A (1987) A proposed nomenclature for the alcohol-soluble proteins (zeins) of maize (Zea mays L.). J Cereal Sci 5:117–128
Esen A, Bietz JA, Paulis JW, Wall JS (1987) A 23.8-kD alphazein with N-terminal sequence and immunological properties similar to 26.7-kD alpha-zeins. Plant Molec Biol 9:421–430
Hartings H, Bonanomi S, Soave C, Di Fonzo N, Salamini F (1984) Mapping of genes for minor zein SDS subunits and revision of zein genes nomenclature. Genet Agrar 38:447–464
Heidecker G, Messing J (1986) Structural analysis of plant genes. Annu Rev Plant Physiol 37:439–466
Immer FR (1930) Formulae and tables for calculating linkage intensities. Genetics 15:81–98
Marks MD, Lindell JS, Larkins BA (1985) Nucleotide sequence analysis of zein mRNAs from maize endosperm. J Biol Chem 260:16451–16459
Nucca R, Soave C, Motto M, Salamini F (1978) Taxonomic significance of the zein isoelectric focusing pattern. Maydica 23:239–249
Ottoboni LMM, Steffensen DM (1987) Localization of zein genes in maize. Biochem Genet 25:123–142
Paulis JW, Wall JS, Sanderson J (1978) Origin of high methionine content in sugary-1 corn endosperm. Cereal Chem 55:705–712
Phillips RL, McClure BA (1985) Elevated protein-bound methionine in seeds of a maize line resistant to lysine plus threonine. Cereal Chem 62:213–218
Rubenstein I, Geraghty DE (1986) The genetic organization of zein. In: Pomeranz Y (ed) Advances in cereal science and technology, vol 8. Am Assoc Cereal Chem, St. Paul/MN, pp 297–315
Sheridan WF (1982) Maps, markers and stocks. In: Sheridan WF (ed) Maize for biological research. Plant Molecular Biology Assoc, Charlottesville/VA, pp 37–52
Smith JSC, Smith OS (1986) Environmental effects on zein chromatograms of maize inbred lines revealed by reversed-phase high-performance liquid chromatography. Theor Appl Genet 71:607–612
Soave C, Salamini F (1984) Organization and regulation of zein genes in maize endosperm. Philos Trans R Soc London, Ser B 304:341–347
Soave C, Reggiani R, Di Fonzo N, Salamini F (1981) Clustering of genes for 20 kd zein subunits in the short arm of maize chromosome 7. Genetics 97:363–377
Soave C, Reggiani R, Di Fonzo N, Salamini F (1982) Genes for zein subunits on maize chromosome 4. Biochem Genet 20:1027–1238
Stuber CW, Goodman MM (1983) Allozyme genotypes for popular and historically important inbred lines of corn, Zea mays L. USDA Agric Res Results, Southern Ser. No. 16. 1–29
Valentini G, Soave C, Ottaviano E (1979) Chromosomal location of zein genes in Zea mays. Heredity 42:33–40
Viotti A, Cairo G, Vitale A, Sala E (1985) Each zein gene class can produce polypeptides of difference sizes. EMBO J 4:1103–1110
Wilson CM (1984) Isoelectric focusing of zein in agarose. Cereal Chem 61:198–200
Wilson CM (1985a) Mapping of zein polypeptides after isoelectric focusing on agarose gels. Biochem Genet 23:115–124
Wilson CM (1985b) A nomenclature for zein polypeptides based on isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis. Cereal Chem 62:361–365
Wilson CM (1986) Serial analysis of zein by isoelectric focusing and sodium dodecyl sulfate gel electrophoresis. Plant Physiol 82:196–202
Wilson CM (1987) Proteins of the kernel. In: Watson SA, Ramstad PE (eds) Corn: Chemistry and technology. Am Assoc Cereal Chemists, St. Paul/MN, pp 273–310
Wilson DR, Larkins BA (1984) Zein gene organizaion in maize and related grasses., J Mol Evol 20:330–340
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Communicated by A. L. Kahler
Cooperative investigations of the U.S. Department of Agriculture, Agricultural Research Service, and the Illinois Agricultural Experiment Station, Department of Agronomy, University of Illinois, Urbana, USA
Mention of firm names or trade products does not imply that they are endorsed or recommended by the U.S. Department of Agriculture over other firms or similar products not mentioned
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Wilson, C.M., Sprague, G.F. & Nelsen, T.C. Linkages among zein genes determined by isoelectric focusing. Theoret. Appl. Genetics 77, 217–226 (1989). https://doi.org/10.1007/BF00266190
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DOI: https://doi.org/10.1007/BF00266190