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Journal of Comparative Physiology B

, Volume 163, Issue 5, pp 355–362 | Cite as

Valine uptake by the scleractinian coral Galaxea fascicularis: characterization and effect of light and nutritional status

  • S. Al-Moghrabi
  • D. Allemand
  • J. Jaubert
Article

Abstract

The characteristics of valine uptake by isolated microcolonies of Galaxea fascicularis (Linnaeus 1758) were studied under various conditions including light, dark and feeding. The results demonstrated the presence of: (1) a linear component which might represent either a diffusional transport or a low-affinity carrier-mediated transport (apparent carrier affinity >250 μmol·l−1), and (2) a high-affinity active carrier-mediated transport (apparent carrier affinity about 5 μmol·l-1). The latter is mediated by two different systems: (i) a Na+-dependent carrier, stimulated by light and operative in both fed and unfed polyps, and (ii) a Na+-independent carrier, light insensitive and present only in unfed polyps. Competition experiments with other amino acids show that the Na+-dependent carrier is highly specific for neutral amino acids, as indicated by the high inhibition constants of basic and acidic amino acids. Our results suggest that the energy supplied by zooxanthellae photosynthates is necessary for the process of amino acid uptake, and that the Na+-dependent carrier responsible for valine uptake by G. fascicularis is similar to the B0,+ system.

Key words

Amino acid uptake Light Feeding Coral, Galaxea fascicularis 

Abbreviations

AA

amino acid(s)

AC/HC

ratio autotrophic/heterotrophic carbon

ASW

artificial sea water

DOM

dissolved organic material

HPLC

high performance liquid chromatography

K1

apparent inhibition constant

Km

apparent affinity of the carrier

SE

standard error

Vmax

maximal rate of absorption

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • S. Al-Moghrabi
    • 1
  • D. Allemand
    • 1
  • J. Jaubert
    • 1
    • 2
  1. 1.Observatoire Océanologique EuropéenCentre Scientifique de MonacoMonaco, Principality of Monaco
  2. 2.Centre de Biologie MarineUniversité de Nice-Sophia AntipolisNice-Cedex 2France

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