Summary
The pepM gene coding for a methionine-specific aminopeptidase was cloned from Salmonella typhimurium and its nucleotide sequence determined. The gene encoded a 264 amino acid protein that was homologous to a similar protein from Escherichia coli. The sequence of an overproducer mutant allele, pepM100, contained a single base change in the likely −35 region of the pepM promoter that increased its homology to the consensus promoter sequence. A region downstream from the pepM coding sequence contained extensive inverted repeats and was homologous to sequences found elsewhere in both Salmonella and other bacterial species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adams JM (1968) On the release of the formyl group from nascent protein. J Mol Biol 33:571–589
Alm RA, Stroeher UH, Manning PA (1988) Extracellular proteins of Vibrio cholerae: nucleotide sequence of the structural gene (hlyA) for the haemolysin of the haemolytic EL Tor strain 017 and characterization of the hlyA mutation in the non-haemolytic classical strain 569B. Mol Microbiol 2:481–488
An G, Bendiak DS, Mamelak LA, Friesen JD (1981) Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts. Nucleic Acids Res 9:4163–4172
Ben-Bassat A, Bauer K, Chang S-Y, Myambo K, Boosman A, Chang S (1987) Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure. J Bacteriol 169:751–757
Bower SG, Hove-Jensen F, Switzer RL (1988) Structure of the gene encoding phosphoribosylpyrophosphate synthetase (prsA) in Salmonella typhimurium. J Bacteriol 170:3243–3248
Chang S-YP, McGary EC, Chang S (1989) Methionine aminopeptidase gene of Escherichia coli is essential for cell growth. J Bacteriol 171:4071–4072
Erickson BD, Burton ZF, Watanabe KK, Burgess RR (1985) Nucleotide sequence of the rpsU-dnaG-rpoD operon from Salmonella typhimurium and a comparison of this sequence with the homologous operon of Escherichia coli. Gene 40:67–78
Frischauf AM, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170:827–842
Hanau R, Koduri RK, Ho N, Brenchley JE (1983) Nucleotide sequence of the control regions from the glnA and glnL genes of Salmonella typhimurium. J Bacteriol 155:82–89
Hoopes BC, McClure WR (1987) Strategies in regulation of transcription initiation. In: Neidhardt FC (ed) Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. American Society for Microbiology, Washington, DC, pp 1231–1240
Lathe R (1985) Synthetic oligonucleotide probes deduced from amino acid sequence data. Theoretical and practical considerations. J Mol Biol 183:1–12
Maniatis T, Fritsch EF, Sambrook J (1982 Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Manulis SD, Kobayashi Y, Keen NT (1988) Molecular cloning and sequencing of a pectate lyase gene from Yersinia pseudotuberculosis. J Bacteriol 170:1825–1830
Maruyama T, Gojobori T, Aota S, Ikemura T (1986) Codon usage tabulated from the GenBank genetic sequence data. Nucleic Acids Res 14:151–197
Miller CG (1987) Protein degradation and proteolytic modification. In: Neidhardt FC (ed) Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. American Society for Microbiology, Washington, DC, pp 680–691
Miller CG, Strauch KL, Krukal AM, Miller JL, Wingfield PT, Mazzei GJ, Werlen RC, Graber P, Movva NR (1987) N-terminal methionine-specific peptidase in Salmonella typhimurium. Proc Natl Acad Sci USA 84:2718–2722
Miller CG, Krukal AM, Miller JL, Movva NR (1989) pepM is an essential gene in Salmonella typhimurium. J Bacteriol 171:5215–5217
Ostrowski J, Jagura-Burdzy G, Kredich NM (1987) DNA sequences of the cysB regions of Salmonella typhimurium and Escherichia coli. J Biol Chem 262:5999–6005
Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448
Plamann LS, Stauffer GV (1987) Nucleotide sequence of the Salmonella typhimurium metR gene and the metR-metE control region. J Bacteriol 169:3932–3937
Post LE, Strycharz GD, Nomura M, Lewis H, Dennis PP (1979) Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli. Proc Nall Acad Sci USA 76:1697–1701
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Stephens PE, Lewis HM, Darlison MG, Guest JR (1983) Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12. Eur J Biochem 135:519–527
Stern MJ, Ames GF-L, Smith NH, Robinson EC, Higgins CF (1984) Repetitive extragenic palindromic sequences: a major component of the bacterial genome. Cell 37:1015–1026
Wingfield P, Graber P, Turcatti G, Movva NR, Pelletier M, Craig S, Rose K, Miller CG (1989) Purification and characterization of a methionine-specific aminopeptidase from Salmonella typhimurium. Eur J Biochem 180:23–32
Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
Zucker M (1989) Computer prediction of RNA structure. Methods Enzymol 180 (in press)
Author information
Authors and Affiliations
Additional information
Communicated by J.W. Lengeler
Rights and permissions
About this article
Cite this article
Rao Movva, N., Semon, D., Meyer, C. et al. Cloning and nucleotide sequence of the Salmonella typhimurium pepM gene. Molec. Gen. Genet. 223, 345–348 (1990). https://doi.org/10.1007/BF00265075
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00265075