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Specialized transduction of the biotin region of Escherichia coli by phage T1

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Summary

Phage T1 transduces Bio+ by special mechanism which leads to a higher efficiency of Bio+ transduction than other bacterial markers. Efficient Bio+ transduction depends on a site located between the galactose operon and the bacterial attachment site for phage λ. Evidence is presented which supports the hypothesis that the site is essential for efficient Bio+ transduction because at the site phage T1 initiates head filling in a polar (unidirectional) fashion leading to increased pickup of the Bio+ marker.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Bowman Gray School of Medicine, 27103, Winston-Salem, North Carolina, USA

    H. Drexler

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  1. H. Drexler
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Communicated by G. Bertani

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Drexler, H. Specialized transduction of the biotin region of Escherichia coli by phage T1. Molec. Gen. Genet. 152, 59–63 (1977). https://doi.org/10.1007/BF00264940

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  • DOI: https://doi.org/10.1007/BF00264940

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