Summary
A strain of Saccharomyces cerevisiae, known to produce multiple isoaccepting forms of several tRNA's which differ from a standard wild type strain, has been studied genetically. The multiple isoaccepting tRNA phenotype behaves as if it is caused by a single recessive mutation. Five tetrads were analyzed and all showed a 2:2 segregation of mutant to wild type profiles for Phe-tRNAPhe. Furthermore, the multiple isoacceptors for the other tRNA's in the mutant strain are probably caused by the same mutation, since Tyr-tRNATyr and Val-tRNAVal also exhibit 2:2 segregation for mutant versus wild type tRNA profiles and the segregation pattern is the same as that for Phe-tRNAPhe.
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Communicated by F. Kaudewitz
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Bell, J.B., Lo, R.Y.C. & Quah, SK. A mutant of Saccharomyces cerevisiae that exhibits multiple isoacceptors for several of its transfer RNAs. Molec. Gen. Genet. 153, 145–151 (1977). https://doi.org/10.1007/BF00264729
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DOI: https://doi.org/10.1007/BF00264729