Summary
5-AU-synchronized cells of Allium cepa L.meristems show increased yields of chromosomal aberrations as well as SCE's. However, although a caffeine post-treatment increases the frequency of 5-AU-induced abnormal ana-telophases, it has a negligible influence on the 5-AU-induced SCE's. These results strongly suggest that the mechanisms through which the 5-AU-induced DNA damage is transformed into chromosomal aberrations and SCE's are at least partly different.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- 5-AU:
-
5-Aminouracil
- FdUrd:
-
5-fluoro-2′-deoxyuridine
- BrdUrd:
-
5-bromo-2′-deoxyuridine
- Thd:
-
thymidine
- Urd:
-
uridine
- SCE's:
-
sister chromatid exchanges
Literature
Basler, A.; Bachmann, U.; Roszinsky-Kocher, G.; Rohrborn, G. (1979): Effects of caffeine on sister chromatid exchanges (SCE) in vivo. Mutat. Res. 59, 209–214
Davidson, D.; Golding, B.G.; Amstrong, S.W. (1978): Increases in nuclear volume and cell size in meristematic cells arrested by 5-aminouracil. Protoplasma 96, 47–57
Diez, J.L.; Gonzalez-Fernandez, A.; Lopez-Saez, J.F. (1976): Mechanism of mitotic synchronization induced by 5-aminouracil. Exp. Cell Res. 98, 79–89
Duncan, R.E.; Woods, P.S. (1953): Some cytological aspects of antagonism in synthesis of nucleic acids. Chromosoma 6, 45–60
Evans, H.J. (1977): What are sister chromatid exchanges? In: Chromosomes Today, vol. 6 (eds.: De la Chapelle, A. and Sorsa, M.), pp. 315–326. Amsterdam: Elsevier/North-Holland Biomedical Press
Jakob, K.M.; Trosko, J.E. (1965): The relation between 5-aminouracil-induced synchronization and DNA synthesis. Exp. Cell Res. 40, 56–67
Kato, H. (1973): Induction of sister chromatid exchanges by UV light and its inhibition by caffeine. Exp. Cell Res. 82, 383–390
Kato, H. (1977): Mechanisms for sister chromatid exchanges and their relation to the production of chromosomal aberrations. Chromosoma 59, 179–191
Kihlman, B.A. (1977): Caffeine and chromosomes. Amsterdam: Elsevier
Kihlman, B.A.; Sturelid, S. (1978): Effects of caffeine on the frequencies of chromosomal aberrations and sister chromatid exchanges induced by chemical mutagens in root tips of Vicia faba. Hereditas 88, 35–41
Mattingly, E. (1966): Differences in mitotic cycle in various tissues of Vicia faba roots as revealed by 5-AU-synchronization of cell division. Exp. Cell Res. 42, 274–280
Nakanishi, Y.; Schneider, E.L. (1979): In vivo sister-chromatid exchange: a sensitive measure of DNA damage. Mutat. Res. 60, 329–337
Palitti, F.; Becchetti, A. (1977): Effect of caffeine on sister chromatid exchanges and chromosomal aberrations induced by mutagen in Chinese Hamster cells. Mutat. Res. 45, 157–159
Perry, P.; Evans, H.J. (1975): Cytological detection of mutagencarcinogen exposure by sister chromatid exchange. Nature 258, 121–125
Perry, P.; Wolff, S. (1974): New Giemsa method for the differential staining of sister chromatids. Nature 251, 156–158
Popescu, N.C.; Amsbaugh, S.C.; Dipaolo, J.A. (1979): The relevance of caffeine post-treatment to SCE incidence induced in Chinese Hamster cells. Mutat. Res. 60, 313–320
Prensky, W.; Smith, H.H. (1965): The mechanism of 5-AU-induced synchrony of cell division in Vicia faba root meristems. J. Cell Biol. 24, 401–414
Sasaki, M. (1977): Sister chromatid exchange and chromatid interchange as possible manifestation of different DNA repair processes. Nature 269, 623–625
Scheuermann, W.; Klafke-Lobsien, G. (1973): On the influence of 5-aminouracil on the cell cycle of root meristems. Exp. Cell Res. 76, 428–436
Schvartzman, J.B.; Postigo, R.; Gutierrez, C. (1979): Analysis of visible light-induced sister chromatid exchanges in 5-bromodeoxyuridine-substituted chromosomes. Chromosoma 74, 317–328
Smith, H.H.; Fussell, C.P.; Kugelman, B.H. (1963): Partial synchronization of nuclear divisions in root meristems with 5-aminouracil. Science 142, 595–596
Tjio, J.H.; Levan, A. (1950): The use of oxiquinoline in chromosome analysis. Ann. Est. Exp. Aula Dei 2, 21–64
Wagenaar, E.B. (1966): High mitotic synchronization induced by 5-aminouracil in root cells of Allium cepa L. Exp. Cell Res. 43, 184–190
Wolff, S.; Bodycote, D.J. (1975): The induction of chromatid deletions in accord with the breakage-and-reunion hypothesis. Mutat. Res. 29, 85–91
Wolff, S.; Bodycote, D.J.; Painter, R.B. (1974): Sister Chromatid exchanges induced in Chinese Hamster cells by UV irradiation at different stages of the cell cycle: The necessity for cells to pass through S. Mutat. Res. 25, 73–81
Author information
Authors and Affiliations
Additional information
Communicated by D. von Wettstein
Rights and permissions
About this article
Cite this article
Schvartzman, J.B., Hernandez, P. Sister chromatid exchanges and chromosomal aberrations in 5-aminouracil-synchronized cells. Theoret. Appl. Genetics 57, 221–224 (1980). https://doi.org/10.1007/BF00264674
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00264674