Summary
Twenty stable variant lines resistant to isonicotinic acid hydrazide (INH), an inhibitor of the conversion of glycine to serine in the glycolate pathway, were isolated in cell cultures initiated from allodihaploid Nicotiana tabacum. Plants were regenerated from 13 of these lines and explants were tested for resistance. For some lines virtually all of the regenerated plants scored as resistant; for others a mixed population of sensitive and resistant plants were obtained. One or more plants from 5 lines were fertile, presumably as a result of spontaneous diploidization of cells in the plant or culture. Callus initiated from the seed progeny of these plants was resistant to INH confirming the characteristic as a stable mutation. Seedlings from all INH-resistant plants were small and slow-growing, but the slow-growth trait could be separated from resistance in backcrosses of hybrids. In one case (line I21) crosses with sensitive lines show the resistant trait in that line to be dominant.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Asada, K.; Saito, K.; Kitoh, S.; Kasai, Z. (1965): Photosynthesis of glycine and serine in green plants. Plant Cell Physiol. 6, 47–59
Bourgin, J.P. (1978): Valine-resistant plants from in vitro selected tobacco cells. Molec. Gen. Genet. 161, 225–230
Burk, L.G. (1970): Green and light-yellow haploid seedlings from anthers of sulfur tobacco. J. Hered. 61, 279
Burk, L.G.; Menser, H.A. (1964):A dominant aurea mutation in tobacco. Tobacco Sci. 8, 101–104
Carlson, P.S. (1973): Methionine sulfoximine-resistant mutants of tobacco. Science 180, 1366–1368
Chaleff, R.S.; Parsons, M.F. (1978a): Direct selection in vitro for herbicide-resistant mutants of Nicotiana tabacum. Proc. Nat. Acad. Sci. (Wash.) 75, 5104–5107
Chaleff, R.S.; Parsons, M.F. (1978b): Isolation of a glycerol-utilizing mutant of Nicotiana tabacum. Genetics 89, 723–728
d'Amato, F. (1975): The problem of genetic stability in plant tissue and cell cultures. In: Crop Genetic Resources for Today and Tomorrow (eds. Frankel, O.H.; Hawkes, J.G.), pp. 333–348 Cambridge (England): Cambridge Univ. Press
Gengenbach, B.G.; Green, C.E.; Donovan, M. (1977): Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures. Proc. Natl. Acad. Sci. (Wash.) 74, 5113–5117
Kasperbauer, M.J.; Collins, G.B. (1972): Reconstitution of diploids from anther-derived haploids in tobacco. Crop Sci. 12, 98–101
Linsmaier, E.M.; Skoog, F. (1965): Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18, 100–127
Maliga, Pal. (1978): Resistance mutants and their use in genetic manipulation. In: Frontiers of Plant Tissue Culture 1978 (Ed. Thorpe, Trevor, A.), pp. 381–392, Calgary, Alberta, Canada: Intern. Assoc. Plant Tissue Culture
Maliga, P.; Sz.-Breznovits, A.; Márton, L.; Joo, F. (1975): NonMendelian streptomycin-resistant tobacco mutant with altered chloroplasts and mitochondria. Nature 255, 401–402
Márton, L.; Maliga, P. (1975): Control of resistance in tobacco cells to 5-bromodeoxyuridine by a simple Mendelian factor. Plant Sci. Lett. 5, 77–81
Meins, F.; Binns, A. (1977): Epigenetic variation of cultured somatic cells: Evidence for gradual changes in the requirement for factors promoting cell division. Proc. Natl. Acad. Sci. (Wash.) 74, 2928–2932
Pritchard, G.G.; Whittingham, C.P.; Griffin, W.J. (1963): The effect of isonicotinyl hydrazide on the photosynthetic incorporation of radioactive CO2 into ethanol-soluble compounds of Chlorella. J. Exp. Bot 14, 281–289
Sunderland, N. (1977): Nuclear cytology. In: Plant Tissue and Cell Culture (Ed. Street, H.E.), pp. 177–205. Berkeley, CA: Univ. Calif. Press
Widholm, J.M. (1977): Selection and characterization of biochemical mutants. In: Plant Tissue Culture and Its Biotechnological Applications (Ed. Barz, W.; Reinhard, E.; Zenk, M.H.)
Zelitch, I. (1966): Increased rate of net photosynthetic carbon dioxide uptake caused by the inhibition of glycolate oxidase. Plant Physiol. 41, 1623–1631
Zelitch, I. (1973): Alternate pathways of glycolate synthesis in tobacco and maize leaves in relation to rates of photorespiration. Plant Physiol. 51, 299–305
Zelitch, I. (1974): The effect of glycidate, an inhibitor of glycolate synthesis on photorespiration and net photosynthesis. Arch. Biochem. Biophys. 163, 367–377
Author information
Authors and Affiliations
Additional information
Communicated by P. Maliga
Rights and permissions
About this article
Cite this article
Berlyn, M.B. Isolation and characterization of isonicotinic acid hydrazide-resistant mutants of Nicotiana tabacum . Theoret. Appl. Genetics 58, 19–26 (1980). https://doi.org/10.1007/BF00264663
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00264663