Summary
Multiple genetic and epigenetic changes were detected within one plant generation at the bz2:: mu1 mutable allele in a population of 118 plants. Loss of somatic instability in bz2::mu1 was usually correlated with methylation of the Mu1 transposable element; in 6 plants, somatic instability was lost as a result of mutations in bz2::mu1. This is a surprisingly high frequency of mutation per allele (2.5%) for the Mutator family, for which germinal revertants occur at a frequency of about 10−4 per gamete. One germinal excision event was found that contained an 8 by deletion, frameshift mutation in Bronze-2. The three other mutants described occurred as a result of abortive transposition, in which 75–77 by deletions were generated at the junction between Bronze-2 and Mu1. We discuss the possible mechanisms, and the role of host factors in abortive transposition in maize.
Similar content being viewed by others
References
Banks JA, Masson P, Fedoroff N (1988) Molecular mechanisms in the developmental regulation of the maize Suppressor-mutator transposable element. Genes Dev 2:1364–1380
Bennetzen JL, Frascasso RP, Morris DW, Robertson DS, Skogen-Hagenson MJ (1987) Concomitant regulation of Mu1 transposition and Mutator activity in maize. Mol Gen Genet 208:57–62
Britt AB, Walbot V (1991) Germinal and somatic products of Mu1 excision from the Bronze-1 gene of Zea mays. Mol Gen Genet In press
Chandler VL, Walbot V (1986) DNA modification of a maize transposable element correlates with loss of activity. Proc Natl Acad Sci USA 83:1767–1771
Chomet PS, Wessler S, Dellaporta SL (1987) Inactivation of the maize transposable element Activator (Ac) is associated with its DNA modification. EMBO J 6:295–302
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA mini-preparation: Version II. Plant Mol Biol Rep 1: 19–21
Fedoroff NV (1989) Maize transposable elements. In: Berg DE, Howe MM (ed) Mobile DNA. American Society for Microbiology, Washington, D.C.
Hudson AD, Carpenter R, S. CE (1990) Phenotypic effects of short-range and aberrant transposition in Antirrhinum majus. Plant Mol Bio 14:835–844
Levy AA, Britt AB, Luehrsen KR, Chandler VL, Warren C, Walbot V (1989) Developmental and genetic aspects of Mutator excision in maize. Dev Genetics 10: 520–531
Levy AA, Walbot V (1990) Regulation of the timing of transposable element excision during maize development. Science 248:1534–1537
Martin C, Lister C (1989) Genome juggling by transposons: Tam3-induced rearrangements in Antirrhinum majus. Dev Genetics 10:438–451
McClintock B (1953) Mutation in maize. Carnegie Inst. Washington Year Book 52:227–237
McLaughlin M, Walbot V (1987) Cloning of a mutable bz2 allele of maize by transposon tagging and differential hybridization. Genetics 117:771–776
Menssen A, Hohmann S, Martin W, Schnable PS, Peterson PA, Saedler H, Gierl A (1990) The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene. EMBO J 9:3051–3057
Nash J, Luehrsen KR, Walbot V (1990) Bronze-2 gene of maize: Reconstruction of a wild-type allele and analysis of transcription and splicing. Plant Cell 2:1039–1049
Robbins T, Carpenter R, Coen ES (1989) A chromosome rearrangement suggests that donor and recipient sites are associated during Tam3 transposition in Antirrhinum majus. EMBO 8:5–13
Robertson DS (1978) Characterization of a Mutator system in maize. Mutat Res 51:21–28
Schnable PS, Peterson PA, Saedler H (1989) The bz-rcy allele of the Cy transposable element system of Zea mays contains a Mu-like insertion. Mol Gen Genet 217:459–463
Schwartz D, Dennis EA (1986) Transposase activity of the Ac controlling element in maize is regulated by its degree of methylation. Mol Gen Genet 205:476–482
Schwarz-Sommer Z, Gierl A, Cuypers H, Peterson PA, Saedler H (1985) Plant transposable elements generate the DNA sequence diversity needed in evolution. EMBO J 4:591–597
Shattuck-Eidens DM, Bell RN, Neuhausen SL, Helentjaris T (1990) DNA sequence variation within maize and melon: Observations from polymerase chain reaction amplification and direct sequencing. Genetics 126:207–217
Sokal RR, Rohlf FJ (1981) Biometry, Second Edition. W.H. Freeman and Company, New York
Taylor LP, Walbot V (1985) A deletion adjacent to the maize transposable element Mu-1 accompanies loss of Adh1 expression. EMBO J 4:869–876
Walbot V (1986) Inheritance of Mutator activity in Zea mays as assayed by somatic instability of the bz2-mu1 allele. Genetics 114:1293–1312
Walbot V, Warren C (1988) Regulation of Mu element copy number in maize lines with an active or inactive transposable element system. Mol Gen Genet 211:27–34
Wessler S, Tarpley A, Purugganan M, Spell M, Okagaki R (1990) Filler DNA is associated with spontaneous deletions in maize. Proc Natl Acad Sci USA 87:8731–8735
Author information
Authors and Affiliations
Additional information
Communicated by H. Saedler
Rights and permissions
About this article
Cite this article
Levy, A.A., Walbot, V. Molecular analysis of the loss of somatic instability in the bz2::mu1 allele of maize. Molec. Gen. Genet. 229, 147–151 (1991). https://doi.org/10.1007/BF00264223
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00264223