Summary
Mutagenic DNA repair is a function of many naturally occurring plasmids belonging to several different incompatibility groups. A DNA probe corresponding to the impCAB operon of the IncIl plasmid TP110, which encodes such functions, was used to investigate the distribution of homologous sequences in both related and unrelated plasmids. Southern blotting was used to demonstrate considerable sequence conservation amongst a number of plasmid types, with imp-related sequences being found on plasmids belonging to the I1, I1/B, B and FIV incompatibility groups. However, no homology was detected amongst plasmids of the N and L/M incompatibility groups, many of which carry functionally similar gene clusters. It appears that sequences determining mutagenic repair functions have been largely conserved within any one incompatibility group, but that significant divergent evolution has occurred between groups.
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References
Bagg A, Kenyon CJ, Walker GC (1981) Inducibility of a gene product required for UV and chemical mutagenesis. Proc Natl Acad Sci USA 78:5749–5753
Bradley DE (1980) Morphological and serological relationships of conjugative pili. Plasmid 4:155–169
Brunner DP, Traxler BA, Holt SM, Crose LL (1986) Enhancement of UV survival, UV- and MMS-mutability, precise excision of Tn10 and complementation of umuC by plasmids of different incompatibility groups. Mutat Res 166:29–37
Couturier M, Bex F, Bergquist PL, Mass WK (1988) Identification and classification of bacterial plasmids. Microbiol Rev 52:375–395
Datta N, Hughes VM (1983) Plasmids of the same Inc groups in Enterobacteria before and after the medical use of antibiotics. Nature 306:616–617
Dowden SB, Strike P (1982) R46-derived recombinant plasmids affecting DNA repair and mutation in E. coli. Mol Gen Genet 186:140–144
Dowden SB, Glazebrook JA, Strike P (1984) UV-inducible UV protection and mutation functions on the I group plasmid TP110. Mol Gen Genet 193:316–321
Elledge SJ, Walker GC (1983) The muc genes of pKM101 are induced by DNA damage. J Bacteriol 155:1306–1315
Falkow S, Guerry P, Hedges RW, Datta N (1974) Polynuclcotide sequence relationships among plasmids of the I compatibility complex. J Gen Microbiol 85:65–76
Glazebrook JA, Grewal KK, Strike P (1986) Molecular analysis of the UV protection and mutation genes carried by the I incompatibility group plasmid TP110. J Bacteriol 168:251–256
Grindley NDF, Humphreys GO, Anderson ES (1973) Molecular studies of R factor compatibility groups. J Bacteriol 115:387–389
Grunstein M, Hogness D (1975) Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72:3961–3965
Hughes VM, Datta N (1983) Conjugative plasmids in bacteria of the “pre-antibiotic era”. Nature 302:725–726
Jacob EA, Shapiro JA, Yamamoto L, Smith DI, Cohen SW, Berg D (1977) Plasmids studied in Escherichia coli and other enteric bacteria. In: Bukhari AL, Shapiro JA, Adhya SL (eds) DNA insertion elements, plasmids and episomes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 601–704
Kato T, Shinoura Y (1977) Isolation and characterisation of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light. Mol Gen Genet 156:121–131
Kitagawa Y, Akaboshi E, Shinagawa H, Horii T, Ogawa H, Kato T (1985) Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli. Proc Natl Acad Sci USA 82:4336–4340
Lodwick D, Owen D, Strike P (1990) DNA sequence analysis of the imp UV protection and mutation operon of the plasmid TP110: Identification of a third gene. Nucleic Acids Res 18:5045–5050
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Molina AM, Babudri N, Tamaro M, Venturini S, Monti-Bragadin C (1979) Enterobacteriacae plasmids enhancing chemical mutagenesis and their distribution among incompatibility groups. FEMS Lett 5:33–37
Perry KL, Walker GC (1982) Identification of plasmid (pKM101)coded proteins involved in mutagenesis and UV resistance. Nature 300:278–281
Perry KL, Elledge SJ, Mitchell BB, Marsh L, Walker GC (1985) umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA and LexA proteins share homology. Proc Natl Acad Sci USA 82:4331–4335
Pinney RJ (1980) Distribution among incompatibility groups of plasmids that confer UV mutability and UV resistance. Mutat Res 72:155–159
Sedgwick SG (1986) Stability and change through DNA repair. In: Kirkwood TBL, Rosenberger RF, Galas DJ (eds) Accuracy in molecular processes. Chapman and Hall, London, pp 233–289
Sedgwick SG, Robson M, Malik F (1988) Polymorphisms in the umuDC region of Escherichia species. J Bacteriol 170:1610–1616
Sedgwick SG, Thomas SM, Hughes VM, Lodwick D, Strike P (1989) Mutagenic DNA repair genes on plasmids from the ‘preantibiotic era’. Mol Gen Genet 218:323–329
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Strike P, Lodwick D (1987) Plasmid genes affecting DNA repair and mutation. J Cell Sci [Suppl] 6:303–321
Upton C, Pinney RJ (1983) Expression of eight unrelated MUC+ plasmids in eleven DNA repair-deficient E. coli strains. Mutat Res 112:251–254
Weber PC, Palchaudhuri S (1986) Incompatibility repressor in a RepA-like replicon of the incF1 plasmid ColV2-94. J Bacteriol 166:1106–1112
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Lodwick, D., Strike, P. Distribution of sequences homologous to the impCAB operon of TP110 among bacterial plasmids of different incompatibility groups. Molec. Gen. Genet. 229, 27–30 (1991). https://doi.org/10.1007/BF00264209
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DOI: https://doi.org/10.1007/BF00264209