Summary
The authors have elaborated a suitable method of detection of PAS-positive substances with chemicals in common use-thiosemicarbazide-both on semi-thick sections and tissue blocks for electron microscopic purposes. The method is sufficiently sensitive even in the presence of only a small amount of PAS-positive substances. They found that the specifity of this method is the same as that in the PAS method used for light microscopy. The structure of the cells remains surprisingly well preserved, especially in comparison with other methods in current use.
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Šťastná, J., Trávník, P. Electron microscopic detection of PAS-positive substances with thiosemicarbazide. Histochemie 27, 63–68 (1971). https://doi.org/10.1007/BF00263369
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DOI: https://doi.org/10.1007/BF00263369