Summary
Two experiments were conducted in the greenhouse to study the interaction between alfalfa cultivars (Medicago sativa L. and M. falcata L.) and strains of Rhizobium meliloti Dang. for acetylene reduction rate, plant height and dry weights of shoot, root and whole plant. Fifteen alfalfa cultivars were inoculated with 10 strains of Rhizobium in Experiment I. Variance component analysis revealed that more than 30% of the total variance was due to alfalfa cultivars for acetylene reduction rate and 26% was accounted for by Rhizobium strains. More than 36% of the total variation was attributed to the interaction between alfalfa cultivars and Rhizobium strains for this character. Twenty-five host cultivars and 11 Rhizobium strains were included in Experiment II. The results also showed that the interaction of alfalfa cultivars and Rhizobium strains contributed the largest portion of the total variation for dry weights of shoot, root and whole plant and acetylene reduction rate. The results clearly demonstrated that the non-additive effects were the major component of variation for these characters associated with nitrogen fixation in alfalfa. Therefore, an effective way of improving nitrogen fixation in alfalfa is to select for a favourable combination of specific Rhizobium strains and alfalfa cultivars.
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References
Aughtry JD Jr (1948) Effect of genetic factors in Medicago on symbiosis with Rhizobium. Cornell Univ Agric Exp Stn, Mem 280
Bordeleau LM, Antoun H, Lachance RA (1977) Effects des souches de Rhizobium meliloti et des coupes successive de la luzerne (Medicago sativa) sur la fixation symbiotique d'axote. Can J Plant Sci 57:433–439
Brockwell J, Hely FW (1966) Symbiotic characteristics of Rhizobium meliloti: an appraisal of the systematic treatment of nodulation and nitrogen fixation interactions between hosts and rhizobia of diverse origins. Aust J Agric Res 17:885–899
Burton JC (1964) The Rhizobium — legume association. In: Gilmour GM, Allen ON (eds) Microbiology and soil fertility. Proc Biol Coll, Oregon State University Press, Corvallis Ore, pp 107–134
Burton JC (1972) Nodulation and symbiotic nitrogen fixation. In: Hanson CH (ed) Alfalfa science and technology. Agronomy 15, Am Soc Agron, Madison Wis, pp 229–246
Burton JC, Wilson PW (1939) Host plant specificity among the Medicago in association with root-nodule bacteria. Soil Sci 47:293–303
Duhigg P, Melton B, Baltensperger A (1978) Selection for acetylene reduction rates in ‘Mesilla’ alfalfa. Crop Sci 18:813–816
Erdman LW, Means UM (1953) Strain variation of Rhizobium meliloti on three varieties of Medicago saliva. Agron J 45:625–629
Gasser H, Guy P, Obaton M, Sikora I (1972) Efficiency of Rhizobium meliloti strains and their effects on alfalfa cultivars. Can J Plant Sci 52:444–448
Gibson AH (1962) Genetic variation in the effectiveness of nodulation of lucerne varieties. Aust J Agric Res 13:388–399
Hardarson G, Jones DG (1979) Effect of temperature on competition amongst strains of Rhizobium trifolii for nodulation of two white clover varieties. Ann Appl Biol 92:229–236
Hardy RWF, Burns RC, Holsten RD (1973) Applications of the acetylene-ethylene assay for measurement of nitrogen fixation. Soil Biol Biochem 5:47–81
Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Circ 347, Calif Agric Exp Stn, Berkeley Calif
Leach GJ (1968) The effectiveness of nodulation of a wide range of lucerne cultivars. Aust J Exp Agric Anim Husb 8:323–326
Mytton LR (1978) Some problems and potentialities in breeding for improved white clover — rhizobium (Trifolium repens — Rhizobium trifolii) symbiosis. Ann Appl Biol 88:445–448
Mytton LR, El-Sherbeeny MH, Lawes DA (1977) Symbiotic variability in Vicia faba. 3. Genetic effects of host plant, Rhizobium strain and of host X strain interaction. Euphytica 26:785–791
Nutman PS (1946) Genetic factors concerned in the symbiosis of clover and nodule bacteria. Nature 57:463–465
Seetin MW, Barnes DK (1977) Variation among alfalfa genotypes for rate of acetylene reduction. Crop Sci 17:783–787
Snedecor GW, Cochran WG (1967) Statistical methods. The Iowa State University Press, Ames Iowa
Tan G-Y (1981) Genetic variation for acetylene reduction rate and other characters in alfalfa. Crop Sci 21:485–488
Tan G-Y, Tan W-K (1981) Net assimilation rate and relative nitrogen assimilation rate in relation to the dry matter production of alfalfa cultivars. Plant Soil 59:185–192
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Communicated by G. S. Khush
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Tan, G.Y., Tan, W.K. Interaction between alfalfa cultivars and Rhizobium strains for nitrogen Fixation. Theoret. Appl. Genetics 71, 724–729 (1986). https://doi.org/10.1007/BF00263270
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DOI: https://doi.org/10.1007/BF00263270