Summary
In vivo prostatic secretion was collected from retired breeder Sprague Dawley rats using a method for isolated perfusion of the rat prostatic urethra. Enzymatic acid phosphatase determination was performed on the collected effluent. Control acid phosphatase secretion was 24.2±2.7 nm over 30 minutes. Intravenous phenylephrine 5 mg/kg stimulated a 10 fold increase in acid phosphatase secretion. The secretion seen with phenylephrine was dose dependent and could be blocked with prazosin, but not yohimbine, atropine, or propranolol. Intravenous β-adrenergic agonist isoproterenol caused no increase in the secretion of rat prostatic acid phosphatese. Intravenous administration of the cholinergic agonist pilocarpine also resulted in a dose dependent rise in acid phosphatase secretion. The stimulation seen could be blocked by atropine but not phentolamine or propranolol. The stimulation of acid phosphatase secretion seen with α1 adrenergic or cholinergic agonists was not additive. Intravenous vasoactive intestinal peptide did not stimulate acid phosphatase secretion nor did it augment the secretion induced by α1 adrenergic or cholinergic agonists. Release of acid phosphatase into rat prostatic exocrine secretion is under both α1 adrenergic and cholinergic control.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aumuller G, Adler G (1979) Experimental studies of apocrine secretion in the dorsal prostatic epithelium of the rat. Cell Tissue Res 198:145–158
Aumuller G, Enderle-Schmidtt U, Seitz J, Muntzing J, Chandler (1987) Ultrastructure and immunohistochemistry of the lateral prostate in aged rats. Prostate 10:245–256
Bruschini H, Schmidt RA, Tanagho EA (1978) Neurologic control of prostatic secretion in the dog. Invest Urol 15:288–290
Carmena MJ, Sancho JI, Prieto JC (1986) Effects of age and androgens upon functional vasoactive intestinal peptide receptors in rat prostatic epithelial cells. Biochem Biophys Acta 888:338–343
Debruyne FMJ, Thomas CMG (1986) Prostaglandins in urology. Urol Int 41:407–410
Farnsworth WE, Lawrence MH (1965) Regulation of prostate secretion in the rat. Proc Soc Exp Biol Med 119:373–376
Flickinger CJ (1972) The fine structure of the interstitial tissue of the rat prostate. Am J Anat 134:107–126
Flickinger CJ (1974) Protein secretion in the rat ventral prostate and the relation of Golgi vesicles, cisternae, and vacuoles, as studied by electron microscope ratioautography. Anat Rec 180:427–448
Gerhardt PG, Mevag B, Tveter KJ, Purvis K (1983) A systematic study of biochemical differences between the lobes of the rat prostate. Int J Andrology 6:553–562
Gosling JA (1983) In: Hinman F (ed) Autonomic innervations of the prostate in benign prostatic hypertrophy. Springer, Berlin Heidelberg New York, pp 349–360
Haaparanta T, Pousette A, Hoegberg B, Gustafsson JA, Glaumann H (1982) Fractionation of the rat ventral prostate with respect to isolation and exocytosis of the prostatic secretion protein. Biochem Biophys Acta 716:79–93
Ichihara I, Kawamura H (1979) The fine structure of ventral prostatic secretory epithelial cells in older rats. Cell Tissue Res 203:181–188
Isaacs JT (1983) Prostatic structure and function in relation to the etiology of prostatic cancer. Prostate 4:351–366
Kachar B, da Silva PP (1980) Freeze-fracture study of rat ventra prostate: secretory mechanism in the epithelial cell. Anat Rec 198:549–565
Kimura M, Ichihara I (1985) Cytochemical studies of acid phosphatases in the rat lateral prostate with special reference to secretory apparatus and lysosome system. Histochemistry 82:519–523
Kitada S, Kumazawa J (1987) Pharmacological characteristics of smooth muscle in benign prostatic hyperplasia and normal prostatic hyperplasia and normal prostatic tissue. J Urol 138:158–163
Lundberg JM (1981) Evidence for coexistence of vasoactive intestinal peptide (VIP) and acetylcholine in neurons of cat exocrine glands. Acta Physiol Scand [Suppl] 496:1–57
Olsen PS, Poulsen SS, Kirkegaaro P (1985) Adrenergic effects on secretion of epidermal growth factor from Brunner's glands. Gut 26:920–927
Smith ER (1969) The secretion of electrolytes by the pilocarpinestimulated canine prostate gland. Proc Soc Exp Biol Med 132:223–226
Smith ER, Ilieuzski U (1985) Secretion of bromide by the canine prostate. Prostate 6:155–162
Smith ER, Miller TB, Pebler T (1983) Transepithelial voltage changes during prostatic secretion in the dog. Am J Physiol 245:F470-F477
Tenniswood M, Bird CE, Clark AF (1976) Acid phosphatases: androgen dependent markers of rat prostate. Can J Biochem 54:350–357
Tenniswood M, Abrahams P, Bird CE, Clark AF (1978) Effects of castration and androgen replacement on acid phosphatase activity in the adult rat prostate gland. J Endocrinol 77:301–308
Tenniswood MP, Abrahams PP, Bird CE, Clark AF (1982) Age-associated changes in acid phosphatase characteristics in the rat ventral prostate and other organs. Arch Andrology 9:283–291
Terracio L, Rule A, Salvato J, Douglas WHJ (1985) Immunofluorescent localization of an androgen-dependent isoenzyme of prostatic acid phosphatase in rat ventral prostate. Anat Rec 213:131–139
Tseng CC, Tseng EE, Sensibar JA, Lee C (1986) Acid phosphatase in the rat prostate: comparison with other organs. Prostate 9:57–64
Vanha-Pertulla T, Niemi R, Helminen HJ (1972) Separate lysosomal and seretory acid phosphatases in the rat ventral prostate. Invest Urol 9:345–352
Author information
Authors and Affiliations
Additional information
Supported by the US Veterans Administration
Rights and permissions
About this article
Cite this article
Jacobs, S.C., Story, M.T. Autonomic control of acid phosphatase exocrine secretion by the rat prostate. Urol. Res. 17, 311–315 (1989). https://doi.org/10.1007/BF00262989
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00262989