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The PS03 gene is involved in error-prone intragenic recombinational DNA repair in Saccharomyces cerevisiae

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Summary

The induction of gene conversion and mitotic crossing-over by photoaddition of psoralens, 254 nm ultraviolet radiation, and nitrogen mustards was determined in diploid cells homozygous for the pso3-1 mutation and in the corresponding wild type of Saccharomyces cerevisiae. For these different agents, the frequency of non-reciprocal events (conversion) is reduced in the pso3-1 mutant compared to the wild type. In contrast, the frequency of reciprocal events (crossing-over) is increased at a range of doses. These observations, together with the block in induced mutagenesis for both reverse and forward mutations previously reported for the pso3-1 mutant, suggest that the PS03 gene product plays a role in mismatch repair of short patch regions. The block in gene conversion in the pso3 homozygous diploid leads, in the case of nitrogen mustards, to specific repair intermediates which are lethal to the cells.

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Authors and Affiliations

  1. Instituto de Biociências, Departamento de Genetica, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Prof. Annes Dias, 112/15 andar, 90001, Porto Alegre RS, Brazil

    Heloisa Helena Rodrigues de Andrade

  2. Institut Curie, Section de Biologic, UA 1292 CNRS, 26 rue d'Ulm, F-75231, Paris Cedex 05, France

    Ethel Moustacchi

  3. Instituto de Biociências, Departamento de Fisiologia, Farmacologia e Biofisica, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Prof. Sarmento Leite, 500, 90049, Porto Alegre RS, Brazil

    Joao Antonio Pêgas Henriques

Authors
  1. Heloisa Helena Rodrigues de Andrade
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  2. Ethel Moustacchi
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  3. Joao Antonio Pêgas Henriques
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Communicated by W. Gajewski

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Rodrigues de Andrade, H.H., Moustacchi, E. & Pêgas Henriques, J.A. The PS03 gene is involved in error-prone intragenic recombinational DNA repair in Saccharomyces cerevisiae . Molec. Gen. Genet. 219, 75–80 (1989). https://doi.org/10.1007/BF00261160

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  • DOI: https://doi.org/10.1007/BF00261160

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