Summary
Anabaena variabilis ATCC 29413 contains two cryptic plasmids. Clones of the smaller (41 kb) plasmid, designated pRDS1, in cosmid vectors were used to construct a physical map. A clone bank of pRDS1 constructed by ligating fragments from aXhoII digest of a pRDS1 cosmid clone into a mobilizable plasmid was used to locate an origin of replication of pRDS1. Because we were unable to cureA. variabilis of pRDS1, the clone bank was transferred by conjugation to another strain ofAnabaena sp., strain M-131. A 5.3 kb fragment of pRDS1 contained all of the sequences necessary for replication inAnabaena sp. strain M-131 as judged by the ability to rescue the hybrid vector from exconjugants in unchanged form after many generations. Hybrid plasmids derived from pRDS1, one bearing genes for luciferase, were also transferred by conjugation toA. variabilis, where they appeared to recombine with pRDS1.
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Borck K, Beggs JD, Brammar WJ, Hopkins AS, Murray NE (1976) The constructionin vitro of transducing derivatives of phage lambda. Mol Gen Genet 146:199–207
Boros I, Posfai G, Venetianer P (1984) High-copy number derivatives of the plasmid cloning vector pBR322. Gene 30:257–260
Boyer HW, Roulland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA inEscherichia coli. J Mol Biol 41:459–472
Buzby JS, Porter RJ, Stevens SE Jr (1983) Plasmid transformation inAgmenellum quadruplicatum PR-6: construction of biphasic plasmids and characterization of their transformation properties. J Bacteriol 154:1446–1450
Chauvat F, De Vries L, Van der Ende A, Van Arkel GA (1986) A host-vector system for gene cloning in the cyanobacteriumSynechocystis PCC6803. Mol Gen Genet 204:185–191
Currier TC, Wolk CP (1979) Characteristics ofAnabaena variabilis influencing plaque formation by cyanophage N-1. J Bacteriol 139:88–92
Currier TC, Haury JF, Welk CP (1977) Isolation and preliminary characterization of auxotrophs of a filamentous cyanobacterium. J Bacteriol 129:1556–1562
Duyvesteyn MGC, Korsuize J, de Waard A, Vonshak A, Wolk CP (1983) Sequence-specific endonucleases in strains ofAnabaena andNostoc. Arch Microbiol 134:276–281
Elhai J, Welk CP (1988a) A versatile class of positive selection vectors, based on the nonviability of palindrome-containing plasmids, that allows cloning into long polylinkers. Gene 68:119–138
Elhai J, Wolk CP (1988 b) Conjugal transfer of DNA to cyanobacteria. Methods Enzymol 167:747–754
Elhai J, Wolk CP (1990) Developmental regulation and spatial pattern of expression of the structural genes for nitrogenase in the cyanobacteriumAnabaena. EMBO J 9:3379–3388
Finnegan J, Sherratt D (1982) Plasmid Co1E1 conjugal mobility: the nature ofbom, a region required incis for transfer. Mol Gen Genet 185:344–351
Flores E, Wolk CP (1985) Identification of facultatively heterotrophic, N2-fixing cyanobacteria able to receive plasmid vectors fromEscherichia coli by conjugation. J Bacteriol 162:1339–1341
Gendel S (1987) Instability of Tn5 inserts in cyanobacterial cloning vectors. J Bacteriol 169:4426–4430
Gendel S, Straus N, Pulleyblank D, Williams J (1983) Shuttle cloning vectors for the cyanobacteriumAnacystis nidulans. J Bacteriol 156:148–154
Hanahan D (1983) Studies on transformation ofEscherichia coli with plasmids. J Mol Biol 166:557–580
Herrero A, Wolk CP (1986) Genetic mapping of the chromosome of the cyanobacterium,Anabaena variabilis. Proximity of the structural genes for nitrogenase and ribulose-bisphosphate carboxylase. J Biol Chem 261:7748–7754
Herrero A, Elhai J, Hohn B, Welk CP (1984) Infrequent cleavage of cloned Anabaena variabilis DNA by restriction endonucleases fromA. variabilis. J Bacteriol 160:781–784 (printer's correction: J Bacteriol 162:858, 1985)
Heusterspreute M, Thi VH, Emery S, Tournis-Gamble S, Kennedy N, Davison J (1985) Vectors with restriction site banks. IV. pJRD184, a 3793-bp plasmid vector having 43 unique cloning sites. Gene 39:299–304
Hohn B (1983) DNA sequences necessary for packaging of bacteriophage λ DNA. Proc Natl Acad Sci USA 80:7456–7460
Horinouchi S, Weisblum B (1982) Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibiotics. J Bacteriol 150:804–814
Hu N-T, Thiel T, Giddings TH, Wolk CP (1981)Anabaena andNostoc cyanophages from sewage settling ponds. Virology 114:236–246
Kuhlemeier CJ, Borrias WE, Van den Hondel CAMJJ, van Arkel GA (1981) Vectors for cloning in cyanobacteria: construction and characterization of two recombinant plasmids capable of transformation toEscherichia coli K12 andAnacystis nidulans R2. Mol Gen Genet 184:249–254
Lambert GR, Carr NG (1982) Rapid small-scale plasmid isolation by several methods from filamentous cyanobacteria. Arch Microbiol 133:122–125
Laudenbach DE, Straus NA, Gendel S, Williams JP (1983) The large endogenous plasmid ofAnacystis nidulans: mapping, cloning and localization of the origin of replication. Mol Gen Genet 192:402–407
Laudenbach DE, Straus NA, Gendel S, Williams JP (1985) Evidence for two distinct origins of replication in the large endogenous plasmid ofAnacystis nidulans R2. Mol Gen Genet 199:300–305
Maldener I, Lockau W, Cai Y, Wolk CP (1991) Calcium-dependent protease of the cyanobacteriumAnabaena: molecular cloning and expression of the gene inEscherichia coli, sequencing and site-directed mutagenesis. Mol Gen Genet 225:113–120
Maly FE, Urwyler A, Rolli HP, Dahinden CA, de Week AL (1988) A single-photon imaging system for the simultaneous quantitation of luminescent emissions from multiple samples. Anal Biochem 168:462–469
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Reaston J, van den Hondel CAMJJ, van Arkel GA, Stewart WDP (1982) A physical map of plasmid pDU1 from the cyanobacteriumNostoc PCC 7524. Plasmid 7:101–104
Schmetterer G, Wolk CP (1988) Identification of the region of cyanobacterial plasmid pDU1 necessary for replication inAnabaena sp. strain M-131. Gene 62:101–109
Shaffer PW, Lockau W, Wolk CP (1978) Isolation of mutants of the cyanobacterium,Anabaena variabilis, impaired in photoautotrophy. Arch Microbiol 117:215–219
Simon RD (1978) Survey of extrachromosomal DNA found in the filamentous cyanobacteria. J Bacteriol 136:414–418
Wolk CP, Vonshak A, Kehoe P, Elhai J (1984) Construction of shuttle vectors capable of conjugative transfer fromEscherichia coli to nitrogen-fixing filamentous cyanobacteria. Proc Natl Acad Sci USA 81:1561–1565
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Murry, M.A., Wolk, C.P. Identification and initial utilization of a portion of the smaller plasmid ofAnabaena variabilis ATCC 29413 capable of replication inAnabaena sp. strain M-131. Molec. Gen. Genet. 227, 113–119 (1991). https://doi.org/10.1007/BF00260715
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DOI: https://doi.org/10.1007/BF00260715