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Molecular analysis of the Bacillus subtilis recF function

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Summary

recF resides between the dnaN and gyrB genes of Bacillus subtilis. The recF15 mutation results in replacement of a glutamate residue in the wild type with a lysine residue in the mutant RecF protein. We investigated the in vivo regulation of recF using a transcriptional fusion to the xylE gene and assaying mRNA production. We found that novobiocin leads to a four-fold induction in recF gene expression, but this is not observed in a gyrB mutant strain. Enhancement of expression of the recF gene in the presence of novobiocin is unrelated to the SOS response. The RecF protein, which has a predicted molecular mass of 42.2 kDa, does not seem to be involved in its own regulation.

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Authors and Affiliations

  1. Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, W-1000, Berlin 33, Germany

    Juan C. Alonso & A. Carola Stiege

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  1. Juan C. Alonso
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  2. A. Carola Stiege
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Communicated by W. Goebel

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Alonso, J.C., Stiege, A.C. Molecular analysis of the Bacillus subtilis recF function. Molec. Gen. Genet. 228, 393–400 (1991). https://doi.org/10.1007/BF00260632

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  • DOI: https://doi.org/10.1007/BF00260632

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