Summary
Attempts have been made to cultivate Telotylenchus loofi on plant callus tissues at different temperature ranges. Although the subjection of cultures to 17–20, 30–35 and 35–40° C temperatures did not prove a handicap in nematode propagation, huge populations could only be obtained at 25–27° C. The temperatures below 17° C and beyond 40° C were found unsuitable for cultivation of T. loofi.
Zusammenfassung
Es wurden Versuche durchgeführt, Telotylenchus loofi an Pflanzen Callusgewebe bei verschiedenen Temperaturbereichen zu vermehren. Zuchttemperaturen zwischen 17 und 20°, 30 und 35° und 35–40° ließen zwar keine wesentliche Beeinträchtigung der Vermehrung erkennen, aber die besten Zuchtbedingungen lagen bei 25–27° C. Temperaturen unter 17° C und über 40° C waren für die Kultur von T. loofi ungeeignet.
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References
Barker, K. R., and K. M. Darling: Reproduction of Aphelenchus avenae on plant tissue culture. Nematologica 11, 162–166 (1965).
Darling, H. M., L. R. Faulkner, and P. Wallendal: Culturing the potato rot nematode. Phytopathology 47, 7 (1957).
Dolliver, J. S., A. C. Hildebrandt, and A. J. Riker: Studies of reproduction of Aphelenchoides ritzemabosi (Schwartz) on plant tissue culture. Nematologica 7, 294–300 (1962).
Feder, W. A.: Aseptic culture of the burrowing nematode Radopholus similis on excised okra root tissues. Phytopathology 48, 392–393 (1958).
Khera, S., and B. M. Zuckerman: Studies on the culturing of certain ectoparasitic nematodes on plant tissue callus. Nematologica 8, 272–274 (1962).
G. C. Bhatnagar, M. G. Tikyani, and N. Kumar: Culturing of Telotylenchus indicus Siddiqi, 1960 on alfalfa callus tissue. Proc. Ind. Sci. Cong. (Abst.) Pt. III, Sect. X, 551 (1967).
Krusberg, L. R.: Studies on the culturing and parasitism of plant parasitic nematodes in particular Ditylenchus dipsaci and Aphelenchoides ritzemabosi on alfalfa callus tissue. Nematologica 6, 181–200 (1961).
Peacock, F. C.: The development of technique for studying the host-parasite relationships of the root knot nematode Meloidogyne incognita under controlled conditions. Nematologica 4, 43–55 (1959).
Tikyani, M. G., and S. Khera: A survey of plant parasitic nematodes around the roots of great millet (Sorghum vulgare Pers.) from Rajasthan, India. Plant Dis. Reptr. 52, 396–398 (1968).
-- -- Studies on the longevity of Telotylenchus loofi in absence of host-plant. Ind. J. Helminth. (In press).
Tiner, J. D.: Cultures of the plant parasitic nematode genus Pratylenchus on sterile excised roots. Exp. Parasit. 9, 121–126 (1960).
Tyler, J.: Reproduction without males in aseptic cultures of the root-knot nematode. Hilgardia 7, 373–388 (1933).
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Tikyani, M.G., Khera, S. & Bhatnagar, G.C. Propagation of Telotylenchus loofi on plant callus tissues. Z. F. Parasitenkunde 32, 369–373 (1969). https://doi.org/10.1007/BF00259649
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DOI: https://doi.org/10.1007/BF00259649