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Light-induced transformation of etioplasts to chloroplasts of barley without transcriptional control of plastid gene expression

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Summary

Transcription in etioplasts and chloroplasts of 5 day old barley plants was compared by hybridization of corresponding plastid run-on transcripts to cloned DNA fragments of small size and in sum representing the complete plastid genome. Hybridization signals reflecting the relative transcription rates of the various DNA fragments were almost identical in etioplasts and chloroplasts. Parallel determinations of the relative steady state concentrations of plastid RNA species showed no correlation between synthesis rate and accumulation of transcripts. Differences in the relative steady state levels of RNAs in etioplasts and chloroplasts were hardly detectable. The results indicate that in barley light-dependent transformation of etioplasts to chloroplasts may proceed without obvious changes in the rate of transcription or the stability of transcripts of plastid genes.

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Abbreviations

AMV:

avian myeloblastosis virus

RNasin:

ribonuclease inhibitor (human placenta)

SDS:

sodium dodecyl sulphate

SSPE:

0.18 M NaCl

10 mM:

sodium hydrogen phosphate

1 mM:

EDTA (ethylenediaminetetraacetic acid)

SSC:

0.15 M NaCl

0.015 M:

sodium citrate

TE:

10 mM tris(hydroxymethyl)aminomethane

1 mM:

EDTA

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Authors and Affiliations

  1. Botanisches Institut der Christian-Albrechts-Universität Kiel, Olshausenstraße 40, D-2300, Kiel, Federal Republic of Germany

    Karin Krupinska & Klaus Apel

Authors
  1. Karin Krupinska
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  2. Klaus Apel
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Communicated by R.G. Herrmann

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Krupinska, K., Apel, K. Light-induced transformation of etioplasts to chloroplasts of barley without transcriptional control of plastid gene expression. Mol Gen Genet 219, 467–473 (1989). https://doi.org/10.1007/BF00259621

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  • DOI: https://doi.org/10.1007/BF00259621

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