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Molecular cloning and expression of multiple cellulase genes of Ruminococcus flavefaciens strain 186 in Escherichia coli

  • Applied Genetics and Regulation
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Summary

Cellulase genes of the ruminant micro-organism Ruminococcus flavefaciens strain 186 have been cloned and expressed in Escherichia coli using the bacteriophage vector λNM1149. Twenty-six clones showed expression of endo-β-1,4-d-glucanases and were divided into four groups according to their insert sizes of approximately 2, 3, 4 or 9 kilobases (kb). Two of the clones with 4 kb inserts also showed exo-β-1,4-d glucanase activity while two clones with 9 kb inserts showed β-glucosidase activity. One of the clones with 9 kb inserts (λM903) showed the activities of all three cellulase activities. In addition, two of the 4 kb-insert clones and one 9 kb-insert clone degraded Avicel (PH101).

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Authors and Affiliations

  1. Department of Biotechnology, Massey University, Palmerston North, New Zealand

    Chung-Ming Huang & Pak-Lam Yu

  2. Biotechnology Divison, Department of Scientific and Industrial Research, Palmerston North, New Zealand

    William J. Kelly & Roderick V. Asmundson

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  1. Chung-Ming Huang
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  2. William J. Kelly
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  3. Roderick V. Asmundson
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  4. Pak-Lam Yu
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Huang, CM., Kelly, W.J., Asmundson, R.V. et al. Molecular cloning and expression of multiple cellulase genes of Ruminococcus flavefaciens strain 186 in Escherichia coli . Appl Microbiol Biotechnol 31, 265–271 (1989). https://doi.org/10.1007/BF00258407

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  • DOI: https://doi.org/10.1007/BF00258407

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