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Isolation and characterization of a novel glycosyl hydrolase family 74 (GH74) cellulase from the black goat rumen metagenomic library

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Abstract

This study aimed to isolate and characterize a novel cellulolytic enzyme from black goat rumen by using a culture-independent approach. A metagenomic fosmid library was constructed from black goat rumen contents and screened for a novel cellulase. The KG37 gene encoding a protein of 858 amino acid residues (92.7 kDa) was isolated. The deduced protein contained a glycosyl hydrolase family 74 (GH74) domain and showed 77% sequence identity to two endo-1,4-β-glucanases from Fibrobacter succinogenes. The novel GH74 cellulase gene was overexpressed in Escherichia coli, and its protein product was functionally characterized. The recombinant GH74 cellulase showed a broad substrate spectrum. The enzyme exhibited its optimum activity at pH 5.0 and temperature range of 20–50 °C. The enzyme was thermally stable at pH 5.0 and at a temperature of 20–40 °C. The novel GH74 cellulase can be practically exploited to convert lignocellulosic biomass to value-added products in various industrial applications in future.

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Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen 21 Program (Project No. PJ011163) of the Rural Development Administration, Republic of Korea. This research was also supported by the Chung-Ang University Research Scholarship Grant in 2015 (Y.-H. Song).

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Authors and Affiliations

  1. Department of Food Science and Technology, Chung-Ang University, Ansung, 456-756, South Korea

    Yun-Hee Song, Jin-Young Baek, Min-Ju Kim & Keun-Sung Kim

  2. Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Wanju, 565-851, South Korea

    Kyung-Tai Lee, Mi-Ra Kwon, Young-Joo Kim, Mi-Rim Park & Haesu Ko

  3. Department of Biological Sciences, Kyonggi University, Suwon, 442-760, South Korea

    Jin-Sung Lee

Authors
  1. Yun-Hee Song
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  2. Kyung-Tai Lee
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  3. Jin-Young Baek
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  4. Min-Ju Kim
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  5. Mi-Ra Kwon
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  6. Young-Joo Kim
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  7. Mi-Rim Park
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  8. Haesu Ko
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  9. Jin-Sung Lee
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  10. Keun-Sung Kim
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Corresponding author

Correspondence to Keun-Sung Kim.

Additional information

Yun-Hee Song and Kyung-Tai Lee contributed equally to this work.

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Song, YH., Lee, KT., Baek, JY. et al. Isolation and characterization of a novel glycosyl hydrolase family 74 (GH74) cellulase from the black goat rumen metagenomic library. Folia Microbiol 62, 175–181 (2017). https://doi.org/10.1007/s12223-016-0486-3

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  • DOI: https://doi.org/10.1007/s12223-016-0486-3

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