Summary
Two experiments were carried out on a dysmoder humus sampled from a depth of 2–5 cm from a mixed hardwood forest. In acid soil, the chloroform fumigation-incubation method failed to estimate the microbial biomass, not because bacterial growth was inhibited after fumigation but because a labile C source was taken up which differed from the killed biomass C.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson JPE, Domsch KH (1978) Mineralization of bacteria and fungi in chloroform-fumigated soils. Soil Biol Biochem 10:207–213
Anderson RV, Coleman DC, Cole CV, Elliott ET (1981) Effect of the nematodes Acrobeloides sp. and Mesodiplogaster Iheritieri on substrate utilization and nitrogen and phosphorus mineralization in soil. Ecology 62:549–555
Chapman SJ (1987) Inoculum in the fumigation method for soil biomass determination. Soil Biol Biochem 19:83–87
Chaussod R, Nicolardot B (1982) Mesure de la biomasse microbienne et estimation simplifiée du carbone facilement mineralisable. Rev Ecol Biol Sol 19:501–512
Coleman DC, Anderson RV, Cole CV, Elliott ET, Woods L, Campion MK (1978) Trophic interactions in soils as they affect energy and nutrient dynamics: IV. Flows of metabolic and biomass carbon. Microb Ecol 4:373–380
Coûteaux MM, Palka L (1988) A direct counting method for soil ciliates. Soil Biol Biochem 20:7–10
Coûteaux MM, Faurie G, Palka L, Steinberg C (1988) La relation prédateur-proie (protozoaires-bactèries) dans les sots: Rô1e dans la régulation des populations et conséquences sur les cycles du carbone et de l'azote. Rev Ecol Biol Sol 25:1–31
Ingham ER, Horton KA (1987) Bacteria, fungal and protozoan responses to chloroform fumigation in stored soil. Soil Biol Biochem 19:545–550
Jenkinson DS (1966) Studies on the decomposition of plant material in soil: II. Partial sterilization of soil and soil biomass. J Soil Sci 17:280–302
Jenkinson DS, Powlson DS (1976) The effects of biocidal treatment on metabolism in soil: V. A method for measuring soil biomass. Soil Biol Biochem 8:209–213
Jenkinson DS, Powlson DS, Wedderburn RWM (1976) The effects of biocidal treatments on metabolism in soil: III. The relationship between soil biovolume measured by optical microscopy, and the flush of decomposition caused by fumigation. Soil Biol Biochem 8:189–202
Jenkinson DS, Davidson SA, Powlson DS (1979) Adenosine triphosphate and microbial biomass in soil. Soil Biol Biochem 11:521–527
Laybourn J, Finlay BJ (1976) Respiratory energy losses related to cell weight and temperature in ciliate protozoa. Oecologia 24:349–355
Martens R (1985) Limitations in the application of the fumigation technique for biomass estimations in amended soils. Soil Biol Biochem 17:57–63
Nicolardot B, Chaussod R, Catroux G (1984) Décomposition de corps microbiens dans des sots fumigés au chloroforme: effets du type de sot et de microorganisme. Soil Biol Biochem 16:453–458
Palka L, Couteaux MM (1986) Aptitude à la croissance de Colpoda aspera (protozoaire cilié) dans un sot humique préalablement stérilisé à l'oxyde de propylène, à l'autoclave et aux rayons gamma. Rev Ecol Biol Sol 23:405–421
Powlson DS, Jenkinson DS (1976) The effects of biocidal treatments on metabolism in soil — II. Gamma irradiation, autoclaving, air-drying and fumigation. Soil Biol Biochem 8:179–188
Steinberg C, Faurie G, Zegerman M, Pave A (1987) Régulation par les protozoaires d'une population bactérienne introduite dans le sot: Modéelisation mathématique de la relation prédateur-proie. Rev Ecol Biol Sol 24:42–62
Vance ED, Brookes PC, Jenkinson DS (1987) Microbial biomass measurements in forest soils: Determination of Kc values and tests of hypotheses to explain the failure of the chloroform fumigation-incubation method in acid soils. Soil Biol Biochem 19:689–696
Voroney RP, Paul EA (1984) Determination of Kc and Kn in situ for calibration of the chloroformfumigation-incubation method. Soil Biol Biochem 16:9–14
Williams BL, Sparling CP (1984) Extractable N and P in relation to microbial biomass in UK acid organic soils. Plant and Soil 76:139–148
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Coûteaux, M.M., Henkinet, R., Pitta, P. et al. Native carbon mineralization of an acid organic soil after use of the chloroform-fumigation method to estimate microbial biomass. Biol Fert Soils 8, 172–177 (1989). https://doi.org/10.1007/BF00257762
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00257762