Abstract
To compare the skeletal distribution of calcium and pyrophosphate complexes, autoradiograms of the growing zone of 10-week-old rabbit femurs were performed. Due to its stability to chemical and enzymatic hydrolysis and to the relatively long half-life of the isotope, 96Tc-labelled pyrophosphate was used. Calcium uptake was studied with carrier free 45Cal. For both tracers, the blood supply plays a significant role by delivering the radiopharmaceuticals. In a second step their partition was governed by mixing in different bone structures: calcium was incorporated inside the bone trabeculae and the mineral mass whereas labelled pyrophosphate remained in linear patterns surrounding bone spicules.
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References
Beaune P (1977) Origine et fixation des pyrophosphates des pariétaux de jeunes rats. Biochimie 59:833–838
Brookes M (1971) The blood supply of bone. Butterworths. London
Cartier P, Lanzetta A (1961) Les consitituants minéraux des tissus calcifiés. VIII-La liaison entre le collagène et le “sel de l'os”. Bull Soc Chim Biol 43:981–991
Fleisch H, Russel RGG, Straumann F (1966) Effect of pyrophosphate on hydroxyapatite and its implications in calcium homeostasis. Nature 212:901–903
Francis MD (1969) The inhibition of calcium hydroxyapatite crystal growth polyphosphonates and polyphosphates. Calcif Tissue Res 3:151–162
Guillemart A, Besnard JC, Le Pape A, Galy G, Fetissoff F (1978) Skeletal uptake of pyrophosphate labelled with technetium-95m and technetium-96, as evaluated by autoradiography. J Nucl Med 19:895–899
Guillemart A, Le Pape A, Galy G, Besnard JC (1980) Bone kinetics of calcium-45 and pyrophosphate labelled with technetium-96. J Nucl Med 21:466–470
Jones AG, Francis MD, Davis MA (1976) Bone scanning: radionuclidic reaction mechanisms. Semin Nucl Med 6:3–18
Kaye M, Silverton S, Rosenthall L (1975) Technetium-99m-pyrophosphate: studies in vivo and in vitro. J Nucl Med 16:40–45
Langevelde van A, Driessen OMJ, Pauwels EKJ, Thesing CW (1977) Aspects of 99m-technetium binding from an ethane-1-hydroxy-1,1-diphosphonate-99mTc complex to bone. Eur J Nucl Med 2:47–51
Rosenthall L, Kaye M (1975) Technetium-99m-pyrophosphate kinetics and imaging in metabolic bone disease. J Nucl Med 16:33–39
Rosenthall L, Kaye M (1976) Observations on the mechanism of 99mTc-labelled phosphate complex uptake in metabolic bone disease. Semin Nucl Med 6:59–67
Russell RGG, Fleisch H (1975) Pyrophosphate and diphosphonates in skeletal metabolism: Physiological, clinical and therapeutic aspects. Clin Orthop 108:241–263
Subramanian G, McAfee JG (1971) A new complex of 99m-Tc for skeletal imaging. Radiology 99:192–196
Tilden RL, Jackson J, Enneking WF, DeLand FH, McVey JT (1973) 99mTc-polyphosphate: Histological localization in human femurs by autoradiography. J Nucl Med 14:576–578
Vaughan JM (1975) Movements of ions in and out of the skeleton. In: The physiology of bone. Clarendon, Oxford; pp 136–164
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Le Pape, A., Guillemart, A. Autoradiographic comparison of 96Tc-pyrophosphate and 45Ca bone uptake. Eur J Nucl Med 7, 127–129 (1982). https://doi.org/10.1007/BF00256400
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DOI: https://doi.org/10.1007/BF00256400