Summary
To study the role of the anterior hypothalamic area (AHA) in the control of the estrous (E) rise of follicle stimulating hormone (FSH), female hamsters were subjected to anterior hypothalamic deafferentation (D) or sham deafferentation (S) on 1500 h of proestrus (P). Serum levels of FSH and luteinizing hormone (LH) were measured by radioimmunoassay and FSH and LH releasing activities measured by bioassay in the anterior (AH) and medial basal hypothalamus (MBH) at several time periods during P and E. D did not affect serum levels of LH, whereas there was an attenuation of FSH levels during P and E. D caused an increase in FSH releasing activity in the AH within 1 h, while LH releasing activity was elevated 8 h after D. In animals with D, LH releasing activity in the MBH was elevated during P and was similar to controls during E. FSH releasing activity in the MBH increased during early E and dropped precipitously by 1400 h of E. Collectively, these data demonstrate dichotomous changes in FSH and LH releasing activities and provide further evidence for dual control mechanisms for these two gonadotropins.
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References
Barraclough CA, Turgeon JL, Cramer OM (1975) Neural correlates of adenohypophyseal LH release in rats. In: Stumpf WE, Grant LD (eds) Anatomical neuroendocrinology. Karger, Basel, pp 200–207
Bast JD, Greenwald GS (1974) Serum profiles of follicle stimulating hormone, luteinizing hormone and prolactin during the estrous cycle of the hamster. Endocrinology 94: 1295–1299
Blask DE, Reiter RJ (1975) The pineal gland of the blind-anosmic female rat: its influence on medial basal hypothalamic LRH, PIF and/or PRF activity in vivo. Neuroendocrinology 17: 362–374
Blask DE, Reiter RJ, Vaughan MK, Johnson LY (1979) Differential effects of the pineal gland on LH-RH and FSH-RH activity in the medial basal hypothalamus of the male golden hamster. Neuroendocrinology 28: 36–43
Brawer JR, Ruf KB, Naftolin F (1980) Effects of estradiol-induced lesions of the arcuate nucleus on gonadotropin release in response to preoptic stimulation in the rat. Neuroendocrinology 30: 144–149
Chappel SC, Barraclough CA (1976) Hypothalamic regulation of pituitary FSH secretion. Endocrinology 98: 927–935
Chappel SC, Barraclough CA (1977) Further studies on the regulation of FSH secretion. Endocrinology 101: 24–31
Chappel SC, Norman RL, Spies HG (1977) Regulation of the second (estrous) release of follicle stimulating hormone in hamsters by the medial basal hypothalamus. Endocrinology 101: 1339–1342
Chappel SC, Norman RL, Spies HG (1979) Evidence for a specific neural event that controls the estrous release of follicle stimulating hormone in golden hamsters. Endocrinology 104: 169–174
Coutifaris C, Chappel SC (1983) Involvement of hypothalamic luteinizing hormone-releasing hormone in the regulation of the estrous follicle stimulating hormone surge in the female golden hamster. Endocrinology 113: 563–568
Curtis A, Fink G (1983) A high molecular weight precursor of luteinizing hormone releasing hormone from rat hypothalamus. Endocrinology 112: 390–392
Huffman GE, Gibbs FP (1982) LHRH pathways in rat brain: “deafferentation” spares a sub-chiasmatic LHRH projection to the median eminence. Neuroscience 7: 1979–1993
Kalra SP, Velasco ME, Sawyer CH (1970) Influences of hypothalamic deafferentation on pituitary FSH release and estrogen feedback in immature female parabiotic rats. Neuroendocrinology 6: 228–235
Kalra SP, Ajika K, Krulich L, Fawcett CP, Quijada M, McCann SM (1971) Effects of hypothalamic and preoptic electrochemical stimulation on gonadotropin and prolactin release in proestrous rats. Endocrinology 88: 1150–1158
Kalra SP, Krulich L, McCann SM (1979) Changes in gonadotropin-releasing factor content in the rat hypothalamus following electrochemical stimulation of anterior hypothalamic area and during the estrous cycle. Neuroendocrinology 12: 321–333
Kalra SP (1976) Ovarian steroids differentially augment pituitary FSH release in deafferented rats. Brain Res 114: 541–544
Kimura F, Kawakami M (1978) Reanalysis of the preoptic afferents and efferents involved in the surge of LH, FSH and prolactin release in the proestrous rats. Neuroendocrinology 27: 74–85
King JC, Anthony ELP, Fitzgerald DM, Stopa EG (1985) Luteinizing hormone-releasing hormone neurons in human preoptic/hypothalamus: differential intraneuronal localization of immunoreactive forms. J Clin Endocr Metab 60: 88–97
Lamperti AA (1985) FSHand LH-releasing activity in the hypothalamus of female hamsters during proestrus and estrus: effects of arcuate nucleus lesions. Neuroendocrinology 41: 374–379
Lamperti AA, Baldwin DM (1983) Effects of anterior hypothalamic deafferentation and arcuate nucleus lesions on the feedback actions of estradiol benzoate on luteinizing hormone and follicle stimulating hormone secretion in the female hamster. Biol Reprod 28: 745–751
Lumpkin MD, McCann SM (1984) Effect of destruction of the dorsal anterior hypothalamus on follicle stimulating hormone secretion in the rat. Endocrinology 115: 2473–2480
Lundanes E, Tsuji K, Rampold G, Ohta M, Folkers K, Bowers CY (1980) Studies on the follicle stimulating hormone releasing hormone by radioimmunoassays and by bioassays. Biochem Biophys Res Commun 94: 827–836
McCann SM, Mizunuma H, Samson WK, Lumpkin MD (1983) Differential hypothalamic control of FSH secretion: a review. Psychoneuroendocrinology 8: 299–308
Millar RP, Aehnelt C, Rossier G (1977) Higher molecular weight immunoreactive species of luteinizing hormone releasing hormone: possible precursors of the hormone. Biochem Biophys Res Commun 74: 720–731
Mizunuma H, Samson WK, Lumpkin MD, McCann SM (1983a) Evidence for an FSH-releasing factor in the posterior median eminence. Life Sci 33: 2003–2009
Mizunuma H, Samson WK, Lumpkin MD, Moltz JH, Fawcett CP, McCann SM (1983b) Purification of a bioactive FSH-releasing factor (FSHRH). Brain Res Bull 10: 623–629
Norman RL, Blake CA, Sawyer CH (1972) Delay of the proestrous ovulatory surge of LH in the hamster by pentobarbital or ether. Endocrinology 91: 1025–1029
Palkovits M, Pattou E, Herman JP, Kordon C (1984) Mapping of LH-RH-containing projections to the mediobasal hypothalamus by differential deafferentation experiments. Brain Res 298: 283–288
Rush ME, Ashiru OA, Blake CA (1982) Effects of complete hypothalamic deafferentation on the estrous phase of follicle stimulating hormone release in the cyclic rat. Biol Reprod 26: 399–403
Seeburg PH, Adelman JP (1984) Characterization of cDNA for precursor of human luteinizing hormone releasing hormone. Nature 311: 666–668
Taketani Y, Nozaki M, Taga M, Minaguchi H, Kigawa T, Sakamoto S, Kobayashi H (1980) Effect of hypothalamic deafferentation on the distribution of luteinizing hormonereleasing hormone (LHRH) in the rat brain. Endocrinol Jpn 27: 297–305
Truppo RM, Goodman RL (1985) Evidence that the anterior hypothalamus contributes to the control of tonic secretion of follicle stimulating hormone in the female rat. Biol Reprod 32: 753–760
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Supported in part by NIH grant RR-5417
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Lamperti, A., Hill, L. The effects of anterior hypothalamic deafferentation on FSH-releasing activity in the intact female hamster. Exp Brain Res 68, 189–194 (1987). https://doi.org/10.1007/BF00255244
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DOI: https://doi.org/10.1007/BF00255244