Abstract
The phase diagram of monolayers of l-α-dimyristoyl phosphatidic acid has been studied by fluorescence microscopy. For pressures corresponding to the nearly horizontal slope in the pressure area diagram the growth of crystalline platelets can be observed. They are of dendritic nature; their sizes can be controlled via pressure, compression speed, temperature and pH, and increased up to 100 μm. Due to repulsive interaction a hexagonal arrangement of crystalline platelets can be established.
It is shown that the textures do not depend on the dye probe for concentrations below 3 mol%. On the other hand via incorporation of impurities in concentrations of about 1 mol% the coexistence of lipid and solid phases can be controlled. Since, for a constant surface pressure, this coexistence can be maintained, these monolayers are suitable model systems to study the interactions of proteins and vesicles with coexisting fluid and solid membrane areas.
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Abbreviations
- DMPA:
-
l-α-dimyristoyl phosphatidic acid
- DPPC:
-
l-α-dipalmitoylphosphatidylchline
- DP-NBD-PE:
-
l-α-dipalmitoyl-nitrobenzoxadiazol-phosphatidylethanolamin
- diO-C18 (3):
-
3,3′-dioctadecyl-oxocarbocyanin
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Lösche, M., Möhwald, H. Impurity controlled phase transitions of phospholipid monolayers. Eur Biophys J 11, 35–42 (1984). https://doi.org/10.1007/BF00253856
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DOI: https://doi.org/10.1007/BF00253856