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Depolarized fluorescence photobleaching recovery

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Abstract

The effects of the fact that the laser sources typically used in fluorescence photobleaching recovery (FPR) experiments in the most commonly employed in-line microscope imaging geometries, are highly linearly polarized, are examined in some detail. The implications of the results, in particular for the interpretation of FPR data in complex cell membrane systems in terms of laterally mobile and immobile sub-populations of the labelled molecular species of concern, are discussed. Methods of experimentally eliminating the potentially major rotational diffusion-based “artifacts”, different from those appropriate to three-dimensional (solution or suspension) systems which require other than in-line geometries, are delineated.

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Abbreviations

FPR:

fluorescence photobleaching recovery

FRAP:

fluorescence recovery after photobleaching

2- and 3-D:

two- and three-dimensional

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  1. Paterson Institute for Cancer Research, Christie Hospital & Holt Radium Institute, M20 9BX, Manchester, UK

    R. E. Dale

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Dale, R.E. Depolarized fluorescence photobleaching recovery. Eur Biophys J 14, 179–193 (1987). https://doi.org/10.1007/BF00253843

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  • DOI: https://doi.org/10.1007/BF00253843

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